Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55373-1-AP targets SH3RF3 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | SH3 domain containing ring finger 3 |
| Calculated molecular weight | 93 kDa |
| GenBank accession number | NM_001099289 |
| Gene Symbol | SH3RF3 |
| Gene ID (NCBI) | 344558 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TEJ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SH3RF3 is a ubiquitously expressed RING finger E3 ligase that belongs to the POSH protein family. SH3RF3 also has a partial CRIB domain that has RAC1-binding activity(PMID: 20696164). SH3RF3 contains four SH3 domains for protein interactions and a ring finger domain that confers E3 ubiquitin ligase activity, possibly targeting itself and other proteins for proteasomal degradation (PMID: 41291187).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SH3RF3 antibody 55373-1-AP | Download protocol |
| IHC protocol for SH3RF3 antibody 55373-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |