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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SH3GLB2 Monoclonal antibody (68325-1-Ig)

    WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SH3GLB2 Monoclonal antibody (68325-1-Ig)

    WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SH3GLB2 Monoclonal antibody (68325-1-Ig)

    IP experiment of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

    IP result of anti-SH3GLB2 (IP:68325-1-Ig, 4ug; Detection:68325-1-Ig 1:800) with HeLa cells lysate 1080 ug. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SH3GLB2 Monoclonal antibody (68325-1-Ig)

    IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SH3GLB2 Monoclonal antibody (68325-1-Ig)

    IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SH3GLB2 Monoclonal antibody (68325-1-Ig)

    IF Staining of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH3GLB2 antibody (68325-1-Ig, Clone: 4B1A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

SH3GLB2 Monoclonal antibody, PBS Only

SH3GLB2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 68325-1-PBS
Clone No.4B1A10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68325-1-PBS targets SH3GLB2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8932

Product name: Recombinant human SH3GLB2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-395 aa of BC014635

Sequence: MDFNMKKLASDAGIFFTRAVQFTEEKFGQAEKTELDAHFENLLARADSTKNWTEKILRQTEVLLQPNPSARVEEFLYEKLDRKVPSRVTNGELLAQYMADAASELGPTTPYGKTLIKVAEAEKQLGAAERDFIHTASISFLTPLRNFLEGDWKTISKERRLLQNRRLDLDACKARLKKAKAAEAKATTVPDFQETRPRNYILSASASALWNDEVDKAEQELRVAQTEFDRQAEVTRLLLEGISSTHVNHLRCLHEFVKSQTTYYAQCYRHMLDLQKQLGRFPGTFVGTTEPASPPLSSTSPTTAAATMPVVPSVASLAPPGEASLCLEEVAPPASGTRKARVLYDYEAADSSELALLADELITVYSLPGMDPDWLIGERGNKKGKVPVTYLELLS

相同性解析による交差性が予測される生物種
Full Name SH3-domain GRB2-like endophilin B2
Calculated molecular weight 395 aa, 44 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC014635
Gene Symbol SH3GLB2
Gene ID (NCBI) 56904
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9NR46
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).

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