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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Bif-1 Polyclonal antibody

Bif-1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 15422-1-AP

Synonyms

Bax interacting factor 1, Bif 1, Bif1, Endophilin B1, SH3GLB1

Validation Data Gallery

Applications:
  • WB analysis of mouse kidney using 15422-1-AP

    WB analysis of mouse kidney using 15422-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 15422-1-AP

    WB analysis of mouse heart using 15422-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 15422-1-AP

    WB analysis of mouse heart using 15422-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 15422-1-AP

    WB analysis of mouse heart using 15422-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 15422-1-AP

    WB analysis of mouse heart using 15422-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse placenta using 15422-1-AP

    WB analysis of mouse placenta using 15422-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse placenta using 15422-1-AP

    WB analysis of mouse placenta using 15422-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 15422-1-AP

    WB analysis of human placenta using 15422-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 15422-1-AP

    WB analysis of mouse kidney using 15422-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 15422-1-AP

    WB analysis of mouse kidney using 15422-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 15422-1-AP

    WB analysis of HEK-293 using 15422-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 15422-1-AP

    WB analysis of HEK-293 using 15422-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 15422-1-AP

    WB analysis of human placenta using 15422-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15422-1-AP

    IHC staining of human kidney using 15422-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15422-1-AP (Bif-1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with Bif-1 antibody included
  • IHC staining of human kidney using 15422-1-AP

    IHC staining of human kidney using 15422-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15422-1-AP (Bif-1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with Bif-1 antibody included
  • IF Staining of HepG2 using 15422-1-AP

    IF Staining of HepG2 using 15422-1-AP

    Immunofluorescent analysis of HepG2 cells, using SH3GLB1 antibody 15422-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, human placenta tissue, mouse heart tissue, mouse placenta tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Bif-1 Ready-To-Use IHC Kit. Bif-1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15422-1-AP targets Bif-1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bif-1 fusion protein Ag7710 相同性解析による交差性が予測される生物種
Full Name SH3-domain GRB2-like endophilin B1
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC007455
Gene symbol SH3GLB1
Gene ID (NCBI) 51100
RRIDAB_2254564
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Bif-1 antibody 15422-1-APDownload protocol
IHC protocol for Bif-1 antibody 15422-1-APDownload protocol
IF protocol for Bif-1 antibody 15422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

Eur J Pharmacol

SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation.

Authors - Anbo Gao
View Article
humanWB

J Exp Clin Cancer Res

SH3GLB1-related autophagy mediates mitochondrial metabolism to acquire resistance against temozolomide in glioblastoma.

Authors - Chia-Hung Chien
View Article
mouseWB

Oncotarget

Autophagy-related gene expression is an independent prognostic indicator of glioma.

Authors - Huixue Zhang
View Article
humanWB,ChIP

BMC Cancer

Role of SH3GLB1 in the regulation of CD133 expression in GBM cells

Authors - Chia-Hung Chien
View Article