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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SH3BGRL3 Polyclonal antibody (12210-1-AP)

    WB analysis using 12210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SH3BGRL3 Polyclonal antibody (12210-1-AP)

    IHC staining of human stomach cancer using 12210-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SH3BGRL3 Polyclonal antibody (12210-1-AP)

    IHC staining of human stomach cancer using 12210-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SH3BGRL3 Polyclonal antibody

SH3BGRL3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12210-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SH3 domain binding protein 1, SH3BGRL3, SH3BP 1, TIP B1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, U-251 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12210-1-AP targets SH3BGRL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH3BGRL3 fusion protein Ag2857 相同性解析による交差性が予測される生物種
Full Name SH3 domain binding glutamic acid-rich protein like 3
Calculated molecular weight 93 aa, 10 kDa
Observed molecular weight10 kDa
GenBank accession numberBC030135
Gene Symbol SH3BGRL3
Gene ID (NCBI) 83442
RRIDAB_10694666
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H299
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SH3BGRL3, SH3 domain-binding glutamic acid-rich-like protein, could act as a modulator of glutaredoxin biological activity. It may play a role in cytoskeleton organization (PMID: 34380438). SH3BGRL3 acts as a potential prognostic biomarker for urothelial carcinoma and a novel binding partner of epidermal growth factor receptor (PMID: 26286913).

Protocols

Product Specific Protocols
WB protocol for SH3BGRL3 antibody 12210-1-APDownload protocol
IHC protocol for SH3BGRL3 antibody 12210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

ACS Chem Biol

Citrullination/Methylation Crosstalk on Histone H3 Regulates ER-Target Gene Transcription.

Authors - Kathleen W Clancy
View Article