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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

SH3BGR Polyclonal antibody

SH3BGR Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21161-1-AP

Synonyms

21 GARP, 21 glutamic acid rich protein, SH3BGR, SH3BGR protein

Validation Data Gallery

Applications:
  • WB analysis of mouse heart using 21161-1-AP

    WB analysis of mouse heart using 21161-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21161-1-AP (SH3BGR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 21161-1-AP

    WB analysis of mouse skeletal muscle using 21161-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21161-1-AP (SH3BGR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 21161-1-AP

    IHC staining of human heart using 21161-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 21161-1-AP

    IHC staining of human heart using 21161-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human skeletal muscle using 21161-1-AP

    IHC staining of human skeletal muscle using 21161-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 21161-1-AP

    IHC staining of human skeletal muscle using 21161-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 21161-1-AP

    IF Staining of HeLa using 21161-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH3BGR antibody (21161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

21161-1-AP targets SH3BGR in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH3BGR fusion protein Ag14739 相同性解析による交差性が予測される生物種
Full Name SH3 domain binding glutamic acid-rich protein
Calculated molecular weight 239 aa, 26 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC006371
Gene symbol SH3BGR
Gene ID (NCBI) 6450
RRIDAB_2878820
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SH3BGR antibody 21161-1-APDownload protocol
IHC protocol for SH3BGR antibody 21161-1-APDownload protocol
IF protocol for SH3BGR antibody 21161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Int J Mol Sci

SH3-Binding Glutamic Acid Rich-Deficiency Augments Apoptosis in Neonatal Rat Cardiomyocytes.

Authors - Anushka Deshpande
  • KD Validated
View Article