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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SH2D3A Polyclonal antibody (15641-1-AP)

    WB analysis of HeLa using 15641-1-AP (same clone as 15641-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SH2D3A Polyclonal antibody (15641-1-AP)

    WB analysis of HepG2 using 15641-1-AP (same clone as 15641-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SH2D3A Polyclonal antibody (15641-1-AP)

    WB analysis of HEK-293 using 15641-1-AP (same clone as 15641-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SH2D3A Polyclonal antibody (15641-1-AP)

    IHC staining of human ovary cancer using 15641-1-AP (same clone as 15641-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15641-1-AP (SH2D3A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SH2D3A Polyclonal antibody (15641-1-AP)

    IF Staining of HeLa using 15641-1-AP (same clone as 15641-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH2D3A antibody (15641-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

SH2D3A Polyclonal antibody, PBS Only

SH2D3A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15641-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

UNQ175/PRO201, SH2 domain-containing protein 3A, NSP1, Novel SH2-containing protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15641-1-PBS targets SH2D3A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8132

Product name: Recombinant human NSP1;SH2D3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC006281

Sequence: MQVPQDGEDLAGQPWYHGLLSRQKAEALLQQNGDFLVRASGSRGGNPVISCRWRGSALHFEVFRVALRPRPGRPTALFQLEDEQFPSIPALVHSYMTGRRPLSQATGAVVSRPVTWQGPLRRSFSEDTLMDGPARIEPLRARKWSNSQPADLAHMGRSREDPAGMEASTMPISALPRTSSDPVLLKAPAPLGTVADSLRASDGQLQAKAPTKPPRTPSFELPDASERPPTYCELVPRVPSVQGTSPSQSCPEPEAPWWEAEEDEEEENRCFTRPQAEISFCPHDAPSCLLGPQNRPLEPQVLHTLRGLFLEHHPGSTALHLLLVDCQATGLLGVTRDQRGNMGVSSGLEL

相同性解析による交差性が予測される生物種
Full Name SH2 domain containing 3A
Calculated molecular weight 576 aa, 63 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC006281
Gene Symbol SH2D3A
Gene ID (NCBI) 10045
RRIDAB_2286019
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRG2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SH2 domain containing 3A (SH2D3A) Is is a novel SH2-containing protein 1 (NSP1) with proline-rich domains and therefore probably involved in the signal-transduction of some ligand-activated tyrosine kinase receptors. Three phosphorylation sites of NSP1 have been found to date. NSP1 may be located at cell membrane play a role in JNK activation.

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