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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SH2B1 Polyclonal antibody (12226-1-AP)

    WB analysis using 12226-1-AP (same clone as 12226-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12226-1-AP (SH2B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using SH2B1 Polyclonal antibody (12226-1-AP)

    IP experiment of HepG2 using 12226-1-AP (same clone as 12226-1-PBS)

    IP result of anti-SH2B1 (IP:12226-1-AP, 4ug; Detection:12226-1-AP 1:1500) with HepG2 cells lysate 1080 ug. This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SH2B1 Polyclonal antibody (12226-1-AP)

    IHC staining of human ovary cancer using 12226-1-AP (same clone as 12226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12226-1-AP (SH2B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SH2B1 Polyclonal antibody (12226-1-AP)

    IHC staining of human skeletal muscle using 12226-1-AP (same clone as 12226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12226-1-AP (SH2B1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SH2B1 Polyclonal antibody (12226-1-AP)

    IHC staining of human skeletal muscle using 12226-1-AP (same clone as 12226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12226-1-AP (SH2B1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SH2B1 Polyclonal antibody (12226-1-AP)

    IF Staining of HeLa using 12226-1-AP (same clone as 12226-1-PBS)

    Immunofluorescent analysis of HeLa cells using 12226-1-AP (SH2B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

SH2B1 Polyclonal antibody, PBS Only

SH2B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12226-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

KIAA1299, Pro-rich, PH and SH2 domain-containing signaling mediator, PSM, SH2 B, SH2 domain-containing protein 1B

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12226-1-PBS targets SH2B1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2863

Product name: Recombinant human SH2B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC010704

Sequence: MVQREELLSFMGAEEAAPDPAGVGRGGGVAGPPSGGGGQPQWQKCRLLLRSEGEGGGGSRLEFFVPPKASRPRLSIPCSSITDVRTTTALEMPDRENTFVVKVEGPSEYIMETVDAQHVKAWVSDIQECLSPGPCPATSPRPMTLPLAPGTSFLTRENTDSLELSCLNHSESLPSQDLLLGPSESNDRLSQGAYGGLSDRPSASISPSSASIAASHFDSMELLPPELPPRIPIEEGPPAGTVHPLSAPYPPLDTPETATGSFLFQGEPEGGEGDQPLSGYPWFHGMLSRLKAAQLALTGGTGSHGVFLVRQSETRRGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQ

相同性解析による交差性が予測される生物種
Full Name SH2B adaptor protein 1
Calculated molecular weight 426 aa, 45 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC010704
Gene Symbol SH2B1
Gene ID (NCBI) 25970
RRIDAB_2877838
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRF2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SH2B1 is a member of the SH2-domain containing mediators family. SH2B1 has been shown to interact with INS receptor, Grb2, TrkA and Janus kinase 2. Variations close to or in the SH2B1 gene have been found to associate with obesity in two large genome wide association studies of body mass index (PMID: 19079260). SH2B1deletions are associated with severe early-onset obesity (PMID: 19966786).

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