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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SGTA Monoclonal antibody (60305-1-Ig)

    WB analysis using 60305-1-Ig (same clone as 60305-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using SGTA Monoclonal antibody (60305-1-Ig)

    WB analysis of NIH/3T3 using 60305-1-Ig (same clone as 60305-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using SGTA Monoclonal antibody (60305-1-Ig)

    WB analysis of Neuro-2a using 60305-1-Ig (same clone as 60305-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using SGTA Monoclonal antibody (60305-1-Ig)

    WB analysis of fetal human brain using 60305-1-Ig (same clone as 60305-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SGTA Monoclonal antibody (60305-1-Ig)

    IHC staining of human liver cancer using 60305-1-Ig (same clone as 60305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60305-1-Ig (SGTA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SGTA Monoclonal antibody (60305-1-Ig)

    IHC staining of human liver cancer using 60305-1-Ig (same clone as 60305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60305-1-Ig (SGTA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SGTA Monoclonal antibody (60305-1-Ig)

    IF Staining of HeLa using 60305-1-Ig (same clone as 60305-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60305-1-Ig (SGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

SGTA Monoclonal antibody, PBS Only

SGTA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60305-1-PBS
Clone No.3B10B11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Alpha SGT, alphaSGT, hSGT, SGT, SGT1, SGTA, UBP, Vpu binding protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60305-1-PBS targets SGTA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1475

Product name: Recombinant human SGTA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC008885

Sequence: MDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKEMPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIELNPANAVYFCNRAAAYSKLGNYAGAVQDCERAICIDPAYSKAYGRMGLALSSLNKHVEAVAYYKKALELDPDNETYKSNLKIAELKLREAPSPTGGVGSFDIAGLLNNPGFMSMASNLMNNPQIQQLMSGMISGGNNPLGTPGTSPSQNDLASLIQAGQQFAQQMQQQNPELIEQLRSQIRSRTPSASNDDQQE

相同性解析による交差性が予測される生物種
Full Name small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
Calculated molecular weight 34 kDa
Observed molecular weight 34-37 kDa
GenBank accession numberBC008885
Gene Symbol SGTA
Gene ID (NCBI) 6449
RRIDAB_2881419
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO43765
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.

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