Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 20825-1-AP (same clone as 20825-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 20825-1-AP (same clone as 20825-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.
WB analysis of L02 using 20825-1-AP (same clone as 20825-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 20825-1-AP (same clone as 20825-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 20825-1-AP (same clone as 20825-1-PBS)
IP result of anti-SGSM3 (IP:20825-1-AP, 4ug; Detection:20825-1-AP 1:2000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 20825-1-AP (same clone as 20825-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20825-1-AP (SGSM3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 20825-1-AP (same clone as 20825-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20825-1-AP (SGSM3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 20825-1-AP (same clone as 20825-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 20825-1-AP (SGSM3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 20825-1-AP (same clone as 20825-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 20825-1-AP (SGSM3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 20825-1-AP (same clone as 20825-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SGSM3 antibody (20825-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 20825-1-AP (same clone as 20825-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SGSM3 antibody (20825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 20825-1-AP (same clone as 20825-1-PBS)
Immunofluorescent analysis of Hela cells, using SGSM3 antibody 20825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SGSM3 Polyclonal antibody, PBS Only

SGSM3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 20825-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

MAP, Merlin associated protein, Merlin-associated protein, RabGAPLP, Rab-GTPase-activating protein-like protein

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20825-1-PBS targets SGSM3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14800 Product name: Recombinant human SGSM3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 400-749 aa of BC008078 Sequence: RTQRRKSTITALLFGEDDLEALKAKNIKQTELVADLREAILRVARHFQCTDPKNCSVELTPDYSMESHQRDHENYVACSRSHRRRAKALLDFERHDDDELGFRKNDIITIVSQKDEHCWVGELNGLRGWFPAKFVEVLDERSKEYSIAGDDSVTEGVTDLVRGTLCPALKALFEHGLKKPSLLGGACHPWLFIEEAAGREVERDFASVYSRLVLCKTFRLDEDGKVLTPEELLYRAVQSVNVTHDAVHAQMDVKLRSLICVGLNEQVLHLWLEVLCSSLPTVEKWYQPWSFLRSPGWVQIKCELRVLCCFAFSLSQDWELPAKREAQQPLKEGVRDMLVKHHLFSWDVDG 相同性解析による交差性が予測される生物種
Full Name	small G protein signaling modulator 3
Calculated molecular weight	749 aa, 85 kDa
Observed molecular weight	85 kDa
GenBank accession number	BC008078
Gene Symbol	SGSM3
Gene ID (NCBI)	27352
RRID	AB_10693680
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96HU1
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Small G protein signaling modulator 3 (SGSM3) is also named as MAP, RABGAPLP and RABGAPLP. SGSM3 is highly correlated with Cx43 in heart functions and that high levels of SGSM3 may induce Cx43 turnover through lysosomal degradation in infarcted rat hearts (PMID: 29577900, PMID: 32271805). SGSM3 has been shown to be associated with small G-protein-coupled receptor signaling (PMID: 29693742). Several studies have shown that SGSM3 is associated with the risk of some cancers, including liver, breast, colorectal and bladder cancers (PMID: 32271805).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 20825-1-AP (same clone as 20825-1-PBS)

WB analysis of mouse brain using 20825-1-AP (same clone as 20825-1-PBS)

WB analysis of L02 using 20825-1-AP (same clone as 20825-1-PBS)

WB analysis of mouse testis using 20825-1-AP (same clone as 20825-1-PBS)

IP experiment of mouse brain using 20825-1-AP (same clone as 20825-1-PBS)

IHC staining of human heart using 20825-1-AP (same clone as 20825-1-PBS)

IHC staining of human heart using 20825-1-AP (same clone as 20825-1-PBS)

IHC staining of human spleen using 20825-1-AP (same clone as 20825-1-PBS)

IHC staining of human spleen using 20825-1-AP (same clone as 20825-1-PBS)

IF Staining of HEK-293 using 20825-1-AP (same clone as 20825-1-PBS)

IF Staining of HeLa using 20825-1-AP (same clone as 20825-1-PBS)