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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SGSM1 Polyclonal antibody

SGSM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21395-1-AP

Synonyms

KIAA1941, RUTBC2, SGSM1

Validation Data Gallery

Applications:
  • WB analysis of mouse testis using 21395-1-AP

    WB analysis of mouse testis using 21395-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21395-1-AP (SGSM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 21395-1-AP

    WB analysis of mouse testis using 21395-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21395-1-AP (SGSM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skin using 21395-1-AP

    IHC staining of mouse skin using 21395-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 21395-1-AP (SGSM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 21395-1-AP

    IHC staining of mouse skin using 21395-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 21395-1-AP (SGSM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21395-1-AP is SGSM1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGSM1 fusion protein Ag16070 相同性解析による交差性が予測される生物種
Full Name small G protein signaling modulator 1
Calculated molecular weight 1148 aa, 130 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC111698
Gene symbol SGSM1
Gene ID (NCBI) 129049
RRIDAB_10859829
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SGSM1 antibody 21395-1-APDownload protocol
IHC protocol for SGSM1 antibody 21395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols