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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SGOL2 Polyclonal antibody (30867-1-AP)

    WB analysis using 30867-1-AP (same clone as 30867-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30867-1-AP (SGOL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30867-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SGOL2 Polyclonal antibody (30867-1-AP)

    IHC staining of mouse testis using 30867-1-AP (same clone as 30867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30867-1-AP (SGOL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30867-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SGOL2 Polyclonal antibody (30867-1-AP)

    IF Staining of A431 using 30867-1-AP (same clone as 30867-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SGOL2 antibody (30867-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30867-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30867-1-PBS targets SGOL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGOL2 fusion protein Ag29656 相同性解析による交差性が予測される生物種
Full Name shugoshin-like 2 (S. pombe)
Calculated molecular weight 1265 aa, 145 kDa
Observed molecular weight180 kDa
GenBank accession numberBC092412
Gene Symbol SGOL2
Gene ID (NCBI) 151246
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ562F6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SGOL2 (Shugoshin 2, also known as SGO2), which is tightly involved in the cell cycle process, has been reported to protect cohesion, sustain the linkage of chromosomes, and regulate kinetochore-microtubule attachment in meiosis and mitosis (PMID: 21273764). SGOL2 has been reported as a differentially expressed gene in various types of cancer, including glioma, hepatocellular cancer, and endometrial cancer (PMID: 36566018).