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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SGLT1 Polyclonal antibody (30861-1-AP)

    WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SGLT1 Polyclonal antibody (30861-1-AP)

    WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SGLT1 Polyclonal antibody (30861-1-AP)

    WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SGLT1 Polyclonal antibody (30861-1-AP)

    WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using SGLT1 Polyclonal antibody (30861-1-AP)

    IP experiment of mouse kidney using 30861-1-AP (same clone as 30861-1-PBS)

    IP result of anti-SGLT1 (IP:30861-1-AP, 4ug; Detection:30861-1-AP 1:400) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SGLT1 Polyclonal antibody (30861-1-AP)

    IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SGLT1 Polyclonal antibody (30861-1-AP)

    IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SGLT1 Polyclonal antibody (30861-1-AP)

    IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using SGLT1 Polyclonal antibody (30861-1-AP)

    IF Staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SGLT1 antibody (30861-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

SGLT1 Polyclonal antibody, PBS Only

SGLT1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 30861-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

SLC5A1, High affinity sodium-glucose cotransporter

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30861-1-PBS targets SGLT1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29338

Product name: Recombinant human SLC5A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 564-643 aa of NM_000343

Sequence: RNSKEERIDLDAEEENIQEGPKETIEIETQVPEKKKGIFRRAYDLFCGLEQHGAPKMTEEEEKAMKMKMTDTSEKPLWRT

相同性解析による交差性が予測される生物種
Full Name solute carrier family 5 (sodium/glucose cotransporter), member 1
Calculated molecular weight 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberNM_000343
Gene Symbol SGLT1
Gene ID (NCBI) 6523
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP13866
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC5A1, also known as SGLT1, is a sodium-dependent glucose transporter (SGLT) family member. SGLT1 couples sugar transport to Na+ gradients across the intestinal brush border (PMID: 8563765). SGLT1 mediates active glucose and galactose absorption in the intestine and renal glucose scavenging. Mutations in SGLT1 cause glucose-galactose malabsorption (GGM) syndrome (PMID: 34880492). SGLT1 is expressed in intestine and endometrial cells (PMID: 2490366, 28974690).

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