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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SGK3 Polyclonal antibody (12699-1-AP)

    WB analysis of HeLa using 12699-1-AP (same clone as 12699-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skin tissue using SGK3 Polyclonal antibody (12699-1-AP)

    WB analysis of mouse skin using 12699-1-AP (same clone as 12699-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using SGK3 Polyclonal antibody (12699-1-AP)

    WB analysis of A375 using 12699-1-AP (same clone as 12699-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using SGK3 Polyclonal antibody (12699-1-AP)

    WB analysis of rat kidney using 12699-1-AP (same clone as 12699-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using SGK3 Polyclonal antibody (12699-1-AP)

    WB analysis of human liver using 12699-1-AP (same clone as 12699-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using SGK3 Polyclonal antibody (12699-1-AP)

    IP experiment of mouse heart using 12699-1-AP (same clone as 12699-1-PBS)

    IP result of anti-SGK3 (IP:12699-1-AP, 4ug; Detection:12699-1-AP 1:500) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SGK3 Polyclonal antibody (12699-1-AP)

    IHC staining of human pancreas cancer using 12699-1-AP (same clone as 12699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12699-1-AP (SGK3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SGK3 Polyclonal antibody (12699-1-AP)

    IHC staining of human cervical cancer using 12699-1-AP (same clone as 12699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SGK3 Polyclonal antibody (12699-1-AP)

    IHC staining of human cervical cancer using 12699-1-AP (same clone as 12699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using SGK3 Polyclonal antibody (12699-1-AP)

    IF Staining of A375 using 12699-1-AP (same clone as 12699-1-PBS)

    Immunofluorescent analysis of A375 cells using 12699-1-AP (SGK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12699-1-PBS targets SGK3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGK3 fusion protein Ag3149 相同性解析による交差性が予測される生物種
Full Name serum/glucocorticoid regulated kinase family, member 3
Calculated molecular weight 496 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC015326
Gene Symbol SGK3
Gene ID (NCBI) 23678
RRIDAB_2188548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53EW6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Serum- and glucocorticoid-inducible kinase 3 (SGK3) is a protein kinase of the AGC family of protein kinase A, protein kinase G, and protein kinase C and functions downstream of phosphatidylinositol 3-kinase (PI3K)(PMID:21084382). It is also named as DKFZp781N0293, CISK, SGK2, SGKL and may be involved in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK3 mediates cell IL-3-dependent survival signals (PMID:12397388). SGK3 has 2 isoforms produced by alternative splicing with the molecular weight of 57 kDa and 53 kDa.