Validation Data Gallery
Tested Applications
| Positive WB detected in | MDA-MB-231 cells, SH-SY5Y cells, U-251 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27444-1-AP targets SGK196 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24922 Product name: Recombinant human SGK196 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 201-350 aa of BC101548 Sequence: VMCDSNDLPKTLSQYLLTSNFSILANDLDALPLVNHSSGMLVKCGHRELHGDFVAPEQLWPYGEDVPFHDDLMPSYDEKIDIWKIPDISSFLLGHIEGSDMVRFHLFDIHKACKSQTPSERPTAQDVLETYQKVLDTLRDAMMSQAREML 相同性解析による交差性が予測される生物種 |
| Full Name | protein kinase-like protein SgK196 |
| Observed molecular weight | 40-50 kDa |
| GenBank accession number | BC101548 |
| Gene Symbol | SGK196 |
| Gene ID (NCBI) | 84197 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H5K3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SGK196, also known as POMK (protein O-mannose kinase), is an enzyme that plays a significant role in multiple biological processes. As a type II transmembrane protein, SGK196 consists of a cytoplasmic tail, one transmembrane helix, and a luminal domain (PMID: 31913260). It is widely expressed in tissues such as skeletal muscle, brain, heart, and kidney. SGK196 has been identified as a valuable marker for melanoma and has been found to suppress the metastasis of basal-like breast cancer cells, suggesting its role in tumorigenesis and cell survival.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SGK196 antibody 27444-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |