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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha Sarcoglycan Polyclonal antibody

Alpha sarcoglycan Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13222-1-AP

Synonyms

50 DAG, 50DAG, A2, adhalin, ADL, Alpha sarcoglycan, Alpha SG, DAG2, DMDA2, Dystroglycan 2, LGMD2D, SCARMD1, SGCA

Validation Data Gallery

Applications:
  • WB analysis of human skeletal muscle using 13222-1-AP

    WB analysis of human skeletal muscle using 13222-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13222-1-AP (Alpha-sarcoglycan antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy SGCA Ready-To-Use IHC Kit. SGCA primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Product Information

13222-1-AP targets Alpha sarcoglycan in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha sarcoglycan fusion protein Ag3924 相同性解析による交差性が予測される生物種
Full Name sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
Calculated molecular weight 387 aa, 43 kDa
GenBank accession numberBC025702
Gene symbol SGCA
Gene ID (NCBI) 6442
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha-sarcoglycan is a member of the sarcoglycan-sarcospan complex (SG-SSPN), which is constituted by α/ɛ-, β-, γ-, and δ-SGs as well as SSPN. Alpha-sarcoglycan gene expression is exclusive of striated muscle and is finely regulated during myogenic differentiation, where the amount of alpha-sarcoglycan transcript is selectively increased after differentiation to myotubes (MTs). Alpha sarcoglycan's disruption causes limb-girdle muscular dystrophy.

Protocols

Product Specific Protocols
WB protocol for Alpha sarcoglycan antibody 13222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols