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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SFXN3 Recombinant antibody (85978-1-RR)

    WB analysis using 85978-1-RR (same clone as 85978-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85978-1-RR (SFXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85978-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using SFXN3 Recombinant antibody (85978-1-RR)

    IF Staining of C6 using 85978-1-RR (same clone as 85978-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C6 cells using SFXN3 antibody (85978-1-RR, Clone: 250485B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85978-1-PBS in a different storage buffer formulation.

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SFXN3 Recombinant antibody, PBS Only

SFXN3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85978-1-PBS
Clone No.250485B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

SFX3, sideroflexin 3, Sideroflexin-3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85978-1-PBS targets SFXN3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SFXN3 fusion protein Ag7203 相同性解析による交差性が予測される生物種
Full Name sideroflexin 3
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000124
Gene Symbol SFXN3
Gene ID (NCBI) 81855
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BWM7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SFXN3, a mitochondrial membrane protein, is a member of sideroflexin family. Serum anti-SFXN3 autoantibody was elevated in early stage of the oral squamous cell carcinoma significantly, indicating its clinical value for diagnosis of oral squamous cell carcinoma.