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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SFXN1 Monoclonal antibody (68024-1-Ig)

    WB analysis using 68024-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68024-1-Ig (SFXN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SFXN1 Monoclonal antibody (68024-1-Ig)

    IF Staining of MCF-7 using 68024-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SFXN1 antibody (68024-1-Ig, Clone: 2D9F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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SFXN1 Monoclonal antibody

SFXN1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68024-1-Ig
Clone No.2D9F1

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IF/ICC, ELISA

Synonyms

FLJ12876, SFXN1, sideroflexin 1, TCC, Tricarboxylate carrier protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, LNCaP cells, MCF-7 cells, HepG2 cells, HeLa cells, Jurkat cells, MOLT-4 cells, Rat brain tissue, Mouse brain tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68024-1-Ig targets SFXN1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SFXN1 fusion protein Ag2985 相同性解析による交差性が予測される生物種
Full Name sideroflexin 1
Calculated molecular weight35.6 kDa
Observed molecular weight32 kDa
GenBank accession numberBC020517
Gene Symbol SFXN1
Gene ID (NCBI) 94081
RRIDAB_2918768
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H9B4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.

Protocols

Product Specific Protocols
WB protocol for SFXN1 antibody 68024-1-IgDownload protocol
IF protocol for SFXN1 antibody 68024-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols