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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SFXN1 Polyclonal antibody (12296-1-AP)

    WB analysis using 12296-1-AP (same clone as 12296-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using SFXN1 Polyclonal antibody (12296-1-AP)

    WB analysis of MCF-7 using 12296-1-AP (same clone as 12296-1-PBS)

    WB result of SFXN1 antibody (12296-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFXN1 transfected MCF-7 cells. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using SFXN1 Polyclonal antibody (12296-1-AP)

    WB analysis of mouse liver using 12296-1-AP (same clone as 12296-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using SFXN1 Polyclonal antibody (12296-1-AP)

    WB analysis of human brain using 12296-1-AP (same clone as 12296-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SFXN1 Polyclonal antibody (12296-1-AP)

    IP experiment of mouse brain using 12296-1-AP (same clone as 12296-1-PBS)

    IP result of anti-SFXN1 (IP:12296-1-AP, 4ug; Detection:12296-1-AP 1:800) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SFXN1 Polyclonal antibody (12296-1-AP)

    IHC staining of human breast cancer using 12296-1-AP (same clone as 12296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12296-1-AP (SFXN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SFXN1 Polyclonal antibody (12296-1-AP)

    IHC staining of human breast cancer using 12296-1-AP (same clone as 12296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12296-1-AP (SFXN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SFXN1 Polyclonal antibody (12296-1-AP)

    IHC staining of human kidney using 12296-1-AP (same clone as 12296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 12296-1-AP (SFXN1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SFXN1 Polyclonal antibody (12296-1-AP)

    IHC staining of human kidney using 12296-1-AP (same clone as 12296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 12296-1-AP (SFXN1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12296-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SFXN1 Polyclonal antibody, PBS Only

SFXN1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12296-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

sideroflexin 1, Sideroflexin-1, TCC, Tricarboxylate carrier protein

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12296-1-PBS targets SFXN1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2985

Product name: Recombinant human SFXN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-236 aa of BC020517

Sequence: MILIGRMSAQVPMNMTITGCMMTFYRTTPAVLFWQWINQSFNAVVNYTNRSGDAPLTVNELGTAYVSATTGAVATALGLNALTKHVSPLIGRFVPFAAVAAANCINIPLMRQRELKVGIPVTDENGNRLGESANAAKQAITQVVVSRILMAAPGMAIPPFIMNTLEKKAFLKRFPWMSAPIQVGLVGFCLVFATPLCCALFPQKSSMSVTSLEAELQAKIQESHPELRRVYFNKGL

相同性解析による交差性が予測される生物種
Full Name sideroflexin 1
Calculated molecular weight35.6 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC020517
Gene Symbol SFXN1
Gene ID (NCBI) 94081
RRIDAB_2185814
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9B4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.

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