Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IP | See 1 publications below |
Product Information
10152-2-AP targets SFRS4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0204 Product name: Recombinant human SFRS4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-180 aa of BC002781 Sequence: MPRVYIGRLSYQARERDVERFFKGYGKILEVDLKNGYGFVEFDDLRDADDAVYELNGKDLCGERVIVEHARGPRRDGSYGSGRSGYGYRRSGRDKYGPPTRTEYRLIVENLSSRCSWQDLKDYMRQAGEVTYADAHKGRKNEGVIEFVSYSDMKRALEKLDGTEVNGRKIRLVEDKPGSR 相同性解析による交差性が予測される生物種 |
| Full Name | splicing factor, arginine/serine-rich 4 |
| Calculated molecular weight | 57 kDa |
| GenBank accession number | BC002781 |
| Gene Symbol | SFRS4 |
| Gene ID (NCBI) | 6429 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08170 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Splicing factor, arginine/serine-rich 4 (SFRS4, synonym: SRP75). SR proteins are a family of proteins that have a common epitope that binds active sites of polymerase II transcription. SRp75 protein, like other SR proteins, contains an N-terminal RNA recognition motif (RRM), a glycine-rich region, an internal region homologous to the RRM, and a long (315-amino-acid) C-terminal domain composed predominantly of alternating serine and arginine residues. Four of the other SR family members have been shown to restore activity to an otherwise splicing-deficient extract. SRp75 together with SRp20 can also complement the splicing-deficient extract. The apparent molecular mass of dephosphorylated SRp75 is 57 kDa.
Publications
| Species | Application | Title |
|---|---|---|
J Virol Negative and positive mRNA splicing elements act competitively to regulate human immunodeficiency virus type 1 vif gene expression. | ||
PLoS One Relationships between hematopoiesis and hepatogenesis in the midtrimester fetal liver characterized by dynamic transcriptomic and proteomic profiles. |