Validation Data Gallery
Tested Applications
| Positive IHC detected in | human cervical cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
This antibody is recommended for IHC.
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
10916-1-AP targets SRp20 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1341 Product name: Recombinant human SRp20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-164 aa of BC000914 Sequence: MHRDSCPLDCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKRSRNRGPPPSWGRRPRDDYRRRSPPPRRRSPRRRSFSRSRSRSLSRDRRRERSLSRERNHKPSRSFSRSRSRSRSNERK 相同性解析による交差性が予測される生物種 |
| Full Name | splicing factor, arginine/serine-rich 3 |
| Calculated molecular weight | 20 kDa |
| GenBank accession number | BC000914 |
| Gene Symbol | SRSF3 |
| Gene ID (NCBI) | 6428 |
| RRID | AB_2185064 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P84103 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SFRS3, also named as SRSF3 and SRP20, belongs to the splicing factor SR family. It may be involved in RNA processing in relation with cellular proliferation and/or maturation. SFRS3 is one of the cognate factors. It is a proto-oncogene critical for cell proliferation and tumor induction and maintenance.The MW of SFRS3 is 20kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SRp20 antibody 10916-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors | ||
Mol Cell Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation Transition. | ||
Front Oncol SRSF3 Expression Serves as a Potential Biomarker for Prognostic and Immune Response in Pan-Cancer. | ||
Pathol Res Pract LINC01210 promotes malignant phenotypes of colorectal cancer through epigenetically upregulating SRSF3. | ||
Rev Port Cardiol Mesenchymal stem cells may alleviate angiotensin II-induced myocardial fibrosis and hypertrophy by upregulating SFRS3 expression |