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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SFRS2 Polyclonal antibody (21212-1-AP)

    WB analysis using 21212-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21212-1-AP (SFRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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SFRS2 Polyclonal antibody

SFRS2 Polyclonal Antibody for WB, ELISA
Cat No. 21212-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Serine/arginine-rich splicing factor 2, SC-35, SC35, SC 35, Protein PR264

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21212-1-AP targets SFRS2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SFRS2 fusion protein Ag15612 相同性解析による交差性が予測される生物種
Full Name splicing factor, arginine/serine-rich 2
Calculated molecular weight 221 aa, 25 kDa
Observed molecular weight30-35 kDa
GenBank accession numberBC070086
Gene Symbol SFRS2
Gene ID (NCBI) 6427
RRIDAB_3085645
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01130
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SFRS2, also named as PR264 and SC-35, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. The antibody has no cross reaction to SFRS2B.

Protocols

Product Specific Protocols
WB protocol for SFRS2 antibody 21212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols