Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33589-1-AP targets SFRS15 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37951 Product name: Recombinant human SFRS15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 991-1147 aa of BC064990 Sequence: FNSGRDQERFGRRSFGNRVENDRERYGNRNDDRDNSNRDRREWGRRSPDRDRHRDLEERNRRSSGHRDRERDSRDRESRREKEEARGKEKPEVTDRAGGNKTVEPPISQVGNVDTASELEKGVSEAAVLKPSEELPAEATSSVEPEKDSGSAAEAPR 相同性解析による交差性が予測される生物種 |
| Full Name | splicing factor, arginine/serine-rich 15 |
| Observed molecular weight | 150 kDa |
| GenBank accession number | BC064990 |
| Gene Symbol | SFRS15 |
| Gene ID (NCBI) | 57466 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O95104 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SCAF4 is an mRNA anti-terminator protein that plays a crucial role in regulating transcription by RNA Polymerase II. It functions by binding to the phosphorylated CTD of Pol II and interacting with splicing factors to suppress premature transcription termination, particularly at genes with complex structures. This activity ensures proper transcriptional readthrough and full-length mRNA production, thereby influencing gene expression critical for cellular homeostasis. Dysregulation of SCAF4-mediated anti-termination is implicated in disrupted mRNA processing and potential disease states.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SFRS15 antibody 33589-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |