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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SFRS14 Polyclonal antibody (12514-1-AP)

    WB analysis using 12514-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12514-1-AP (SFRS14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SFRS14 Polyclonal antibody (12514-1-AP)

    IHC staining of human prostate cancer using 12514-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12514-1-AP (SFRS14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SFRS14 Polyclonal antibody (12514-1-AP)

    IHC staining of human prostate cancer using 12514-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12514-1-AP (SFRS14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SFRS14 Polyclonal antibody

SFRS14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12514-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

KIAA0365, SFRS14

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, LNCaP cells, rat testis tissue, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12514-1-AP targets SFRS14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3195

Product name: Recombinant human SFRS14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 639-988 aa of BC020586

Sequence: WKARRATTGTQTLLSSGTRLKHHGRQAPGLSQAKPSLPDRNDAAKDCPPDPVGPSPQDPSLEASGPSPKPAGVDISEAPQTSSPCPSADIDMKTMETAEKLARFVAQVGPEIEQFSIENSTDNPDLWFLHDQNSSAFKFYRKKVFELCPSICFTSSPHNLHTGGGDTTGSQESPVDLMEGEAEFEDEPPPREAELESPEASSGTCFPRKRISSKSLKVGMIPAPKRVCLIQEPKVHEPVRIAYDRPRGRPMSKKKKPKDLDFAQQKLTDKNLGFQMLQKMGWKEGHGLGSLGKGIREPVSVYAAGSLGWEWVGPQSFHLQPAAWLLHSQDGLQLAVDFCFLNRRHLQMRS

相同性解析による交差性が予測される生物種
Full Name splicing factor, arginine/serine-rich 14
Calculated molecular weight 988 aa, 120 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC020586
Gene Symbol SFRS14
Gene ID (NCBI) 10147
RRIDAB_2184923
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IX01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SFRS14 antibody 12514-1-APDownload protocol
IHC protocol for SFRS14 antibody 12514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols