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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SFRS11 Recombinant antibody (83605-6-RR)

    WB analysis using 83605-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83605-6-RR (SFRS11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SFRS11 Recombinant antibody (83605-6-RR)

    IF Staining of HepG2 using 83605-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SFRS11 antibody (83605-6-RR, Clone: 240465B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SFRS11 Recombinant antibody (83605-6-RR)

    IF Staining of HepG2 using 83605-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SFRS11 antibody (83605-6-RR, Clone: 240465B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using SFRS11 Recombinant antibody (83605-6-RR)

    FC experiment of HeLa using 83605-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SFRS11 Recombinant antibody (83605-6-RR, Clone:240465B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83605-6-RR

    Affinity and Kinetic Characterization of 83605-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83605-6-RR against Human SFRS11 were performed. The affinity constant is 1.37 nM.

SFRS11 Recombinant monoclonal antibody

SFRS11 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83605-6-RR
Clone No.240465B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

SRSF11, Splicing factor, arginine/serine-rich 11, Serine/arginine-rich splicing factor 11, p54, Arginine-rich 54 kDa nuclear protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HepG2 cells, THP-1 cells, HeLa cells, K-562 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83605-6-RR targets SFRS11 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag24932

Product name: Recombinant human SFRS11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 398-483 aa of BC040436

Sequence: KKKSKDKEKDRERKSESDKDVKVTRDYDEEEQGYDSEKEKKEEKKPIETGSPKTKECSVEKGTGDSLRESKVNGDDHHEEDMDMSD

相同性解析による交差性が予測される生物種
Full Name splicing factor, arginine/serine-rich 11
Calculated molecular weight 483 aa, 53 kDa
Observed molecular weight68 kDa
GenBank accession numberBC040436
Gene Symbol SFRS11
Gene ID (NCBI) 9295
RRIDAB_3671218
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ05519
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRS11 (Serine/arginine-rich splicing factor 11) may function in pre-mRNA splicing. It is localized in the cell nucleus and can colocalize with spliceosome components.

Protocols

Product Specific Protocols
IF protocol for SFRS11 antibody 83605-6-RRDownload protocol
WB protocol for SFRS11 antibody 83605-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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