Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human skin cancer tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 10 publications below |
| IHC | See 8 publications below |
| IF | See 2 publications below |
Product Information
15328-1-AP targets SFRP4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6909 Product name: Recombinant human SFRP4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-346 aa of BC058911 Sequence: MFLSILVALCLWLHLALGVRGAPCEAVRIPMCRHMPWNITRMPNHLHHSTQENAILAIEQYEELVDVNCSAVLRFFLCAMYAPICTLEFLHDPIKPCKSVCQRARDDCEPLMKMYNHSWPESLACDELPVYDRGVCISPEAIVTDLPEDVKWIDITPDMMVQERPLDVDCKRLSPDRCKCKKVKPTLATYLSKNYSYVIHAKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQRRTVQDKKKTAGRTSRSNPPKPKGKPPAPKPASPKKNIKTRSAQKRTNPKRV 相同性解析による交差性が予測される生物種 |
| Full Name | secreted frizzled-related protein 4 |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | 41-51 kDa, 55 kDa |
| GenBank accession number | BC058911 |
| Gene Symbol | SFRP4 |
| Gene ID (NCBI) | 6424 |
| RRID | AB_2187099 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6FHJ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRP4 functions as a suppressor of tumor growth via inhibition of the Wnt signaling pathway.Downregulation or deletion of SFRP4 has been reported in several cancers and cell lines such as endometrial cancer, ovarian cancer, lung cancer,and others. This antibody got 41-51 kDa and 55 kDa bands in western blotting maybe due to differential posttranslational protein modification.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SFRP4 antibody 15328-1-AP | Download protocol |
| IP protocol for SFRP4 antibody 15328-1-AP | Download protocol |
| WB protocol for SFRP4 antibody 15328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Myofibroblast transcriptome indicates SFRP2hi fibroblast progenitors in systemic sclerosis skin. | ||
Clin Transl Med Spatial transcriptomics reveals prognosis-associated cellular heterogeneity in the papillary thyroid carcinoma microenvironment | ||
J Invest Dermatol Fibroblast subpopulations in Systemic Sclerosis - Functional implications of individual subpopulations and correlations with clinical features | ||
Life Sci Short peptide domains of the Wnt inhibitor sFRP4 target ovarian cancer stem cells by neutralizing the Wnt β-catenin pathway, disrupting the interaction between β-catenin and CD24 and suppressing autophagy | ||
Gene Ther Effect of nanoparticle-mediated delivery of SFRP4 siRNA for treating Dupuytren disease.
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