Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28271-1-AP targets SFRP1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28072 Product name: Recombinant human SFRP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 154-314 aa of BC036503 Sequence: MLKCDKFPEGDVCIAMTPPNATEASKPQGTTVCPPCDNELKSEAIIEHLCASEFALRMKIKEVKKENGDKKIVPKKKKPLKLGPIKKKDLKKLVLYLKNGADCPCHQLDNLSHHFLIMGRKVKSQYLLTAIHKWDKKNKEFKNFMKKMKNHECPTFQSVFK 相同性解析による交差性が予測される生物種 |
| Full Name | secreted frizzled-related protein 1 |
| Calculated molecular weight | 314 aa, 35 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC036503 |
| Gene Symbol | SFRP1 |
| Gene ID (NCBI) | 6422 |
| RRID | AB_2881100 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N474 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SFRP1 (Secreted Frizzled-Related Protein 1) is a secreted glycoprotein that functions as a key modulator of the Wnt signaling pathway. The SFRP1 protein harbors two independent structural domains, namely the carboxy-terminal netrin (NTR) domain and an amino-terminal cysteine-rich domain (CRD). Secreted SFRP1 is an inhibitor of the Wnt signalling pathway and has been classified as a tumor suppressor gene due to the loss of its expression in many human cancers (PMID: 32074995). SFRP1 is necessary for stress-induced senescence caused by these factors and is sufficient for the induction of senescence phenotypes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SFRP1 antibody 28271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |