Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, SH-SY5Y cells, U2OS cells, mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 11 publications below |
| IHC | See 6 publications below |
| IF | See 6 publications below |
Product Information
26460-1-AP targets SFRP1 in WB, IHC, IF/ICC, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24844 Product name: Recombinant human SFRP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 164-250 aa of BC036503 Sequence: DVCIAMTPPNATEASKPQGTTVCPPCDNELKSEAIIEHLCASEFALRMKIKEVKKENGDKKIVPKKKKPLKLGPIKKKDLKKLVLYL 相同性解析による交差性が予測される生物種 |
| Full Name | secreted frizzled-related protein 1 |
| Calculated molecular weight | 314 aa, 35 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC036503 |
| Gene Symbol | SFRP1 |
| Gene ID (NCBI) | 6422 |
| RRID | AB_2880522 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N474 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SFRP1 (Secreted Frizzled-Related Protein 1) is a secreted glycoprotein that functions as a key modulator of the Wnt signaling pathway. The SFRP1 protein harbors two independent structural domains, namely the carboxy-terminal netrin (NTR) domain and an amino-terminal cysteine-rich domain (CRD). Secreted SFRP1 is an inhibitor of the Wnt signalling pathway and has been classified as a tumor suppressor gene due to the loss of its expression in many human cancers (PMID: 32074995). SFRP1 is necessary for stress-induced senescence caused by these factors and is sufficient for the induction of senescence phenotypes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SFRP1 antibody 26460-1-AP | Download protocol |
| IHC protocol for SFRP1 antibody 26460-1-AP | Download protocol |
| WB protocol for SFRP1 antibody 26460-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Spatiotemporal interplay between epithelial and mesenchymal cells drives human dentinogenesis. | ||
Genes Dis Naked cuticle homolog 2 controls the differentiation of osteoblasts and osteoclasts and ameliorates bone loss in ovariectomized mice | ||
Cell Rep Med Schwann cell-secreted frizzled-related protein 1 dictates neuroinflammation and peripheral nerve degeneration after neurotrauma | ||
J Orthop Translat Silencing SFRP1 in bone mesenchymal stem cells alleviates pediatric B-ALL-driven bone loss by activating Wnt/β-catenin signaling. | ||
J Transl Med USP22 suppresses trophoblast cell necroptosis in preeclampsia by stabilizing KAT2A-mediated histone acetylation at the SFRP1 promoter. | ||
Int J Mol Med METTL3 inhibits LINC00312 to suppress osteoporosis progression in a YTHDF2‑dependent manner. |