Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, MCF-7 cells |
| Positive IHC detected in | human skin tissue, human breast cancer tissue, human breast hyperplasia tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
66251-1-Ig targets 14-3-3 Sigma in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | 14-3-3 Sigma fusion protein Ag12644 相同性解析による交差性が予測される生物種 |
| Full Name | stratifin |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 28-30 kDa |
| GenBank accession number | BC002995 |
| Gene Symbol | 14-3-3 Sigma |
| Gene ID (NCBI) | 2810 |
| RRID | AB_2881639 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P31947 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for 14-3-3 Sigma antibody 66251-1-Ig | Download protocol |
| IHC protocol for 14-3-3 Sigma antibody 66251-1-Ig | Download protocol |
| IF protocol for 14-3-3 Sigma antibody 66251-1-Ig | Download protocol |
| FC protocol for 14-3-3 Sigma antibody 66251-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis. | ||
Mol Biotechnol Stratifin (SFN) Regulates Cervical Cancer Cell Proliferation, Apoptosis, and Cytoskeletal Remodeling and Metastasis Progression Through LIMK2/Cofilin Signaling | ||
Transl Androl Urol Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples. | ||
Environ Toxicol Multidimensional progressive single-cell sequencing reveals cell microenvironment composition and cancer heterogeneity in lung cancer |