Validation Data Gallery
Tested Applications
| Positive WB detected in | IMR-32 cells, mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23631-1-AP targets SFMBT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20417 Product name: Recombinant human SFMBT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 99-313 aa of BC014614 Sequence: IRKADLYPIGWCEQNKKTLEAPEGIRDKVSDWDEFLRQTLIGACSPPVPLLEGLRNGRNPLDLIAPGSRLECQAFQDSLSTWIVTVVENIGGRLKLRYEGLESSDNYEHWLYYLDPFLHHVGWAAQQGYELQPPSAIRHLKNEAEWQEILAKVKEEEEEPLPSYLFKDKQVIGIHTFSVNMKLEAVDPWSPFGISPATVVKVFDEKYFLVEMDDL 相同性解析による交差性が予測される生物種 |
| Full Name | Scm-like with four mbt domains 1 |
| Calculated molecular weight | 866 aa, 98 kDa |
| Observed molecular weight | 93-98 kDa |
| GenBank accession number | BC014614 |
| Gene Symbol | SFMBT1 |
| Gene ID (NCBI) | 51460 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9UHJ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Scm-like with four MBT domains protein 1 (SFMBT1) is also named as Renal ubiquitous protein 1 (RU1). SFMBT1 is a transcriptional repressor that associates with chromatin and interacts with the N-terminal tail of histone H3 (PMID: 17599839). SFMBT1 forms a complex with Lys-specific demethylase 1 (LSD1) and they are believed to act together in combination with RNA polymerase II in the transcriptional regulation of histone genes (PMID: 23592795). SFMBT1 is up-regulated in CRC tumor tissues and cells and may be associated with drug resistance (PMID: 35577773). SFMBT1 regulates the expression and interacts with key genes involved in heart muscle and artery development (PWWP2A, as well as myogenic differentiation (MYOD, SIX2) (PMID: 35483874). Testes, endocrine glands, and blood are tissues that highly express SFMBT1 (PMID: 25613900). SFMBT1 is also highly expressed in skeletal muscles specifically in undifferentiated myoblasts, and this expression starts diminishing during muscle differentiation (PMID: 23349461).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SFMBT1 antibody 23631-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |