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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SF4 Polyclonal antibody

SF4 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15614-1-AP

Synonyms

F23858, RBP, RNA binding protein RBP, SF4, splicing factor 4

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 15614-1-AP

    WB analysis of HEK-293 using 15614-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 15614-1-AP

    WB analysis of HEK-293 using 15614-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 15614-1-AP

    WB analysis of mouse testis using 15614-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 15614-1-AP

    IHC staining of human testis using 15614-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15614-1-AP (SF4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 15614-1-AP

    IHC staining of human testis using 15614-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15614-1-AP (SF4 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 15614-1-AP

    IF Staining of HepG2 using 15614-1-AP

    Immunofluorescent analysis of () fixed HepG2 cells using 15614-1-AP (SF4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15614-1-AP targets SF4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SF4 fusion protein Ag8006 相同性解析による交差性が予測される生物種
Full Name splicing factor 4
Calculated molecular weight 72 kDa
Observed molecular weight72 kDa
GenBank accession numberBC002986
Gene symbol SF4
Gene ID (NCBI) 57794
RRIDAB_10859818
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SF4 antibody 15614-1-APDownload protocol
IHC protocol for SF4 antibody 15614-1-APDownload protocol
IF protocol for SF4 antibody 15614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols