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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SF3B1 Recombinant antibody (85640-4-RR)

    WB analysis using 85640-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85640-4-RR (SF3B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85640-4-RR

    Affinity and Kinetic Characterization of 85640-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85640-4-RR against Human SF3B1 were performed. The affinity constant is 6.07 nM.

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SF3B1 Recombinant antibody

SF3B1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85640-4-RR
Clone No.243145C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PRP10, SAP 155, SAP155, SF3b155

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, U2OS cells, Jurkat cells, K-562 cells, HeLa cells, NIH/3T3 cells, C6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85640-4-RR targets SF3B1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SF3B1 fusion protein Ag26689 相同性解析による交差性が予測される生物種
Full Name splicing factor 3b, subunit 1, 155kDa
Calculated molecular weight 146 kDa
Observed molecular weight146 kDa
GenBank accession numberNM_012433
Gene Symbol SF3B1
Gene ID (NCBI) 23451
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Protocols

Product Specific Protocols
WB protocol for SF3B1 antibody 85640-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols