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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SF3A1 Recombinant antibody (86170-2-RR)

    WB analysis using 86170-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86170-2-RR (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using SF3A1 Recombinant antibody (86170-2-RR)

    WB analysis of rat kidney using 86170-2-RR

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 86170-2-RR (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SF3A1 Recombinant antibody (86170-2-RR)

    IF Staining of HeLa using 86170-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SF3A1 antibody (86170-2-RR, Clone: 250768F4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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SF3A1 Recombinant antibody

SF3A1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86170-2-RR
Clone No.250768F4

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

PRP21, SAP 114, SAP114, SF3a120, Spliceosome-associated protein 114

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, rat kidney tissue, HEK-293 cells, K-562 cells, HL-60 cells, LNCaP cells, Neuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86170-2-RR targets SF3A1 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SF3A1 fusion protein Ag8196 相同性解析による交差性が予測される生物種
Full Name splicing factor 3a, subunit 1, 120kDa
Calculated molecular weight 793 aa, 89 kDa
Observed molecular weight120 kDa
GenBank accession numberBC007684
Gene Symbol SF3A1
Gene ID (NCBI) 10291
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15459
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )

Protocols

Product Specific Protocols
WB protocol for SF3A1 antibody 86170-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols