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Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells, HL-60 cells, LNCaP cells, Neuro-2a cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86170-1-RR targets SF3A1 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | SF3A1 fusion protein Ag8196 相同性解析による交差性が予測される生物種 |
Full Name | splicing factor 3a, subunit 1, 120kDa |
Calculated molecular weight | 793 aa, 89 kDa |
Observed molecular weight | 120 kDa |
GenBank accession number | BC007684 |
Gene Symbol | SF3A1 |
Gene ID (NCBI) | 10291 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q15459 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )
Protocols
Product Specific Protocols | |
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WB protocol for SF3A1 antibody 86170-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |