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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEZ6L Recombinant monoclonal antibody (87923-1-RR)

    WB analysis using 87923-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87923-1-RR (Sez6l antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SEZ6L Recombinant monoclonal antibody (87923-1-RR)

    WB analysis using 87923-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87923-1-RR (SEZ6L antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SEZ6L Recombinant monoclonal antibody (87923-1-RR)

    IF Staining of mouse brain using 87923-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SEZ6L antibody (87923-1-RR, Clone: 260140D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SEZ6L Recombinant monoclonal antibody (87923-1-RR)

    IF Staining of mouse brain using 87923-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SEZ6L antibody (87923-1-RR, Clone: 260140D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SEZ6L Recombinant monoclonal antibody

SEZ6L Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 87923-1-RR
Clone No.260140D4

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

Acupuncture-induced protein 1-L, Aig1l, Brain-specific receptor-like protein B, BSRP-B, Kiaa0927

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, mouse spinal cord tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87923-1-RR targets SEZ6L in WB, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg9193

Product name: Recombinant mouse Sez6l protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 32-897 aa of NM_001253916.2

Sequence: EDGGPEGEMHPSTAYLLPSASLESSLEEGVTSAAPGLLPSQEALEAMEESLPPALPDEASVQHTPALRKGLPSLKQLNSARRQLRPLATPTTLQRLGSPASATTKLREPEDPEQPTAPAPLQIAPFTALATTLPHSPQPAQAPDDSSPGSPLDKGDNELTGSASEESQETTTSTIVTTTIITTEQAPALCGVSFSDPEGYIDSSDFPPQPYSSFLECTYNVTVYTGYGVELQVKSVNLSEGELLSIRGVDGPTLTVLANQTLLVEGQVIRSPTNTISVYFRTFQDDGLGTFQLHYQAFMLSCPFPRRPDAGEVTVMDLHSGGVAHFHCHLGYELQGAKTLTCINASKPHWSSQEPVCSAPCGGAVHNATIGRVLSPSFPGTANGSQLCVWTIEAPEGQKLHLHLERLLLHEKDRMIVYSGRTNTSALLYDSLRTESVPFEGLLSEGSSIRIEFTSDQGQAASAFNIRFEAFEKGHCYEPYIQNGNFTTSDPTYNIGTIVEFTCDPGHSLEQGPAVIECVNVRDPYWNDTEPLCRAMCGGELSAVAGVVLSPNWPEPYAEGEDCVWKIHVGEEKRIFLDIQFLNLSNSDILTIYDGDEVVPHVLGQYFGHSSPQKLYSSTPDLTIQFHSDPAGLIFGKGQGFIMNYIEVSRNDSCSDLPEIQNGWKTTSHTELVRGARITYQCDPGYDIVGSDTLTCQWDLSWSSDPPFCEKIMYCTDPGEVEHSTRLISDPVLLVGTTIQYTCSPGFVLEGSSLLTCYSRETGTPIWTSRLPHCVSEESLACDNPGLPENGYQILYKRLYLPGESLTFMCYEGFELMGEVTIRCILGQPSHWSGPLPICKVNQDSFEHALEAEAAAESSLEGGNMA

相同性解析による交差性が予測される生物種
Full Name seizure related 6 homolog like
Calculated molecular weight105 kDa
Observed molecular weight150-200 kDa
GenBank accession numberNM_001253916.2
Gene Symbol Sez6l
Gene ID (NCBI) 56747
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ6P1D5-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The BSRPs (brain-specific receptor-like proteins) were predominantly expressed in the soma and dendrites of neurons and localized in the endoplasmic reticulum (ER). Seizure 6-like protein (SEZ6L, also known as BSRP-B) is a member of the BSRP family. It is a candidate tumor suppressor gene and may contribute to specialized endoplasmic reticulum functions in neurons (PMID: 16814779). The observed molecular weight of SEZ6L is higher than its calculated one, because of N-glycosylation (PMID: 27716410).

Protocols

Product Specific Protocols
IF protocol for SEZ6L antibody 87923-1-RRDownload protocol
WB protocol for SEZ6L antibody 87923-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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