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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SETD2 Polyclonal antibody (55377-1-AP)

    WB analysis using 55377-1-AP (same clone as 55377-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55377-1-AP (SETD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using SETD2 Polyclonal antibody (55377-1-AP)

    WB analysis of U2OS cells using 55377-1-AP (same clone as 55377-1-PBS)

    WB result of SETD2 antibody (55377-1-AP, 1:500) with siRNA transfected U2-OS cells. This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SETD2 Polyclonal antibody (55377-1-AP)

    WB analysis of Jurkat using 55377-1-AP (same clone as 55377-1-PBS)

    WB result of SETD2 antibody (55377-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and SETD2 knockout Jurkat cells. This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using SETD2 Polyclonal antibody (55377-1-AP)

    IP experiment of Jurkat using 55377-1-AP (same clone as 55377-1-PBS)

    IP result of anti-SETD2 (IP:55377-1-AP, 4ug; Detection:55377-1-AP 1:800) with Jurkat cells lysate 1360 ug. This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SETD2 Polyclonal antibody (55377-1-AP)

    IHC staining of human colon cancer using 55377-1-AP (same clone as 55377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55377-1-AP (SETD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using SETD2 Polyclonal antibody (55377-1-AP)

    IHC staining of human oesophagus cancer using 55377-1-AP (same clone as 55377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 55377-1-AP (SETD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SETD2 Polyclonal antibody (55377-1-AP)

    IF Staining of HepG2 using 55377-1-AP (same clone as 55377-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETD2 antibody (55377-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SETD2 Polyclonal antibody, PBS Only

SETD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 55377-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:2.1.1.-, EC:2.1.1.359, HIF 1, HIF1, HIF-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55377-1-PBS targets SETD2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name SET domain containing 2
Calculated molecular weight 288 kDa
Observed molecular weight 287 kDa
GenBank accession numberNM_014159
Gene Symbol SETD2
Gene ID (NCBI) 29072
RRIDAB_2881318
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYW2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SETD2, also named as HIF1, HYPB, KIAA1732, KMT3A, SET2, p231HBP, hSET2 and HIP-1, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is a histone methyltransferase that methylates 'Lys-36' of histone H3. SETD2 probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. It binds DNA at promoters. SETD2 may also act as a transcription activator that binds to promoters.(PMID:16118227) It is a potential tumor suppressor gene in breast cancer.(PMID:20944102) This antibody is specific to SETD2.

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