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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using SETD1B Polyclonal antibody (55005-1-AP)

    WB analysis of human brain using 55005-1-AP (same clone as 55005-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using SETD1B Polyclonal antibody (55005-1-AP)

    WB analysis of mouse liver using 55005-1-AP (same clone as 55005-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using SETD1B Polyclonal antibody (55005-1-AP)

    WB analysis of mouse liver using 55005-1-AP (same clone as 55005-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using SETD1B Polyclonal antibody (55005-1-AP)

    WB analysis of MCF-7 using 55005-1-AP (same clone as 55005-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using SETD1B Polyclonal antibody (55005-1-AP)

    IP experiment of MCF-7 using 55005-1-AP (same clone as 55005-1-PBS)

    IP result of anti-SETD1B (IP:55005-1-AP, 5ug; Detection:55005-1-AP 1:300) with MCF-7 cells lysate 3250ug. This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SETD1B Polyclonal antibody (55005-1-AP)

    IHC staining of human breast cancer using 55005-1-AP (same clone as 55005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SETD1B Polyclonal antibody (55005-1-AP)

    IHC staining of human ovary tumor using 55005-1-AP (same clone as 55005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SETD1B Polyclonal antibody (55005-1-AP)

    IHC staining of human ovary tumor using 55005-1-AP (same clone as 55005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SETD1B Polyclonal antibody (55005-1-AP)

    IHC staining of human breast cancer using 55005-1-AP (same clone as 55005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SETD1B Polyclonal antibody (55005-1-AP)

    IHC staining of human breast cancer using 55005-1-AP (same clone as 55005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SETD1B Polyclonal antibody (55005-1-AP)

    IHC staining of human breast cancer using 55005-1-AP (same clone as 55005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SETD1B Polyclonal antibody (55005-1-AP)

    IHC staining of human ovary tumor using 55005-1-AP (same clone as 55005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SETD1B Polyclonal antibody (55005-1-AP)

    IHC staining of human breast cancer using 55005-1-AP (same clone as 55005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SETD1B Polyclonal antibody (55005-1-AP)

    IF Staining of HepG2 using 55005-1-AP (same clone as 55005-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETD1B antibody (55005-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SETD1B Polyclonal antibody, PBS Only

SETD1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 55005-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:2.1.1.364, Histone-lysine N-methyltransferase SETD1B, hSET1B, KIAA1076, KMT2G

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55005-1-PBS targets SETD1B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name SET domain containing 1B
Calculated molecular weight 209 kDa
Observed molecular weight 250-260 kDa, 450 kDa
GenBank accession numberNM_015048
Gene Symbol SETD1B
Gene ID (NCBI) 23067
RRIDAB_10858389
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPS6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SETD1B, also named as KMT2G. Set1B associates with a about 450 kDa complex that contains all five non-catalytic components of the Set1A complex, including CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. The complex is a histone methyltransferase that produces trimethylated histone H3 at Lys4. Set1B is widely expressed, and localize to a largely non-overlapping set of euchromatic nuclear speckles, suggesting that Set1B bind to a unique set of target genes and thus make non-redundant contributions to the epigenetic control of chromatin structure and gene expression( PMID: 17355966). The antibody is specific to SETD1B and, in westernbloting experiments, two specific bands can be detected, about 250 kDa, 450 kDa respectively, in addition, a unknown band around 70 kDa. This antibody is a rabbit polyclonal antibody raised against a region of human SETD1B.

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