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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOLO/SESTD1 Polyclonal antibody (23911-1-AP)

    WB analysis using 23911-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23911-1-AP (SOLO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SOLO/SESTD1 Polyclonal antibody (23911-1-AP)

    WB analysis using 23911-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23911-1-AP (SOLO/SESTD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SOLO/SESTD1 Polyclonal antibody (23911-1-AP)

    IP experiment of mouse brain using 23911-1-AP

    IP result of anti-SOLO (IP:23911-1-AP, 4ug; Detection:23911-1-AP 1:4000) with mouse brain tissue lysate 1280 ug.

  • Immunoprecipitation (IP) experiment of rat brain tissue using SOLO/SESTD1 Polyclonal antibody (23911-1-AP)

    IP experiment of rat brain using 23911-1-AP

    IP result of anti-SOLO (IP:23911-1-AP, 0ug; Detection:23911-1-AP 1:4000) with rat brain tissue lysate 1120 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SOLO/SESTD1 Polyclonal antibody (23911-1-AP)

    IF Staining of HepG2 using 23911-1-AP

    Immunofluorescent analysis of HepG2 cells using 23911-1-AP (SOLO antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using SOLO/SESTD1 Polyclonal antibody (23911-1-AP)

    IF Staining of Jurkat using 23911-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SOLO antibody (23911-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

SOLO/SESTD1 Polyclonal antibody

SOLO/SESTD1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 23911-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

SESTD1, SOLO, Huntingtin-interacting protein-like protein, Protein Solo, SEC14 and spectrin domains 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue, rat brain tissue, L02 cells
Positive IP detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inJurkat cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23911-1-AP targets SOLO/SESTD1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21016

Product name: Recombinant human SESTD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 348-696 aa of BC047578

Sequence: DLVELKSLQQQLSDVCYRQASQLEFRQNLLQAALEFHGVAQDLSQQLDGLLGMLCVDVAPADGASIQQTLKLLEEKLKSVDVGLQGLREKGQGLLDQISNQASWAYGKDVTIENKENVDHIQGVMEDMQLRKQRCEDMVDVRRLKMLQMVQLFKCEEDAAQAVEWLSELLDALLKTHIRLGDDAQETKVLLEKHRKFVDVAQSTYDYGRQLLQATVVLCQSLRCTSRSSGDTLPRLNRVWKQFTIASEERVHRLEMAIAFHSNAEKILQDCPEEPEAINDEEQFDEIEAVGKSLLDRLTVPVVYPDGTEQYFGSPSDMASTAENIRDRMKLVNLKRQQLRHPEMVTTES

相同性解析による交差性が予測される生物種
Full Name SEC14 and spectrin domains 1
Calculated molecular weight 696 aa, 79 kDa
Observed molecular weight 70-79 kDa
GenBank accession numberBC047578
Gene Symbol SOLO
Gene ID (NCBI) 91404
RRIDAB_2879360
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ86VW0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEC14 domain and spectrin repeat-containing protein 1 (SESTD1) is also named Huntingtin-interacting protein-like protein and SOLO. SESTD1, a previously uncharacterized lipid-binding protein strongly expressed in aorta, was found to interact with both TRPC proteins and modulate receptor-activated TRPC5 currents in a recombinant cell line (PMID: 20164195). SESTD1 is proposed to be involved in phospholipid regulation of transient receptor potential channels TRPC4 and TRPC5 (PMID: 26503763). SESTD1 expression is associated with high abundance of immune infiltration, including T helper cells, Th2 cells, and Macrophages in HCC (PMID: 41016345).

Protocols

Product Specific Protocols
IF protocol for SOLO/SESTD1 antibody 23911-1-APDownload protocol
IP protocol for SOLO/SESTD1 antibody 23911-1-APDownload protocol
WB protocol for SOLO/SESTD1 antibody 23911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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