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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse heart tissue using SERTAD1 Polyclonal antibody (10167-1-AP)

    IHC staining of mouse heart using 10167-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10167-1-AP (SERTAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SERTAD1 Polyclonal antibody (10167-1-AP)

    IHC staining of mouse heart using 10167-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10167-1-AP (SERTAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SERTAD1 Polyclonal antibody (10167-1-AP)

    IHC staining of mouse embryo using 10167-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 10167-1-AP (SERTAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SERTAD1 Polyclonal antibody (10167-1-AP)

    IHC staining of mouse embryo using 10167-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 10167-1-AP (SERTAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SERTAD1 Polyclonal antibody (10167-1-AP)

    IHC staining of mouse embryo using 10167-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 10167-1-AP (SERTAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SERTAD1 Polyclonal antibody (10167-1-AP)

    IHC staining of mouse embryo using 10167-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 10167-1-AP (SERTAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SERTAD1 Polyclonal antibody

SERTAD1 Polyclonal Antibody for IHC, ELISA
Cat No. 10167-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

CDK4 binding protein p34SEI1, SEI 1, SEI1, SERTA domain containing 1, SERTAD1, TRIP Br1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse heart tissue, mouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10167-1-AP targets SERTAD1 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERTAD1 fusion protein Ag0180 相同性解析による交差性が予測される生物種
Full Name SERTA domain containing 1
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC002670
Gene Symbol SERTAD1
Gene ID (NCBI) 29950
RRIDAB_2285697
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHV2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERTA domain containing 1 (SERTAD1, synonyms: SEI1, TRIP-Br1) protein is a cyclin-dependent kinase 4 (cdk4)-binding protein and acts as a growth factor sensor and facilitates the formation and activation of cyclin D-CDK complexes in the face of inhibitory levels of INK4 proteins, therefore, antagonizes the activity of p16(INK4a) tumor suppressor. SERTAD1 also suppresses CREB-mediated transcription and regulates cell cycle progression through sequential effects on the transcriptional activity of E2F-responsive promoters during G(1) and S phases.

Protocols

Product Specific Protocols
IHC protocol for SERTAD1 antibody 10167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols