Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse heart tissue, mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10167-1-AP targets SERTAD1 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0180 Product name: Recombinant human SERTAD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-197 aa of BC002670 Sequence: MLSKGLKRKREEEEEKEPLAVDSWWLDPGHTAVAQAPPAVASSSLFDLSVLKLHHSLQQSEPDLRHLVLVVNTLRRIQASMAPAAALPPVPSPPAAPSVADNLLASSDAALSASMASLLEDLSHIEGLSQAPQPLADEGPPGRSIGGAAPSLGALDLLGPATGCLLDDGLEGLFEDIDTSMYDNELWAPASEGLKPG 相同性解析による交差性が予測される生物種 |
| Full Name | SERTA domain containing 1 |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC002670 |
| Gene Symbol | SERTAD1 |
| Gene ID (NCBI) | 29950 |
| RRID | AB_2285697 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHV2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SERTA domain containing 1 (SERTAD1, synonyms: SEI1, TRIP-Br1) protein is a cyclin-dependent kinase 4 (cdk4)-binding protein and acts as a growth factor sensor and facilitates the formation and activation of cyclin D-CDK complexes in the face of inhibitory levels of INK4 proteins, therefore, antagonizes the activity of p16(INK4a) tumor suppressor. SERTAD1 also suppresses CREB-mediated transcription and regulates cell cycle progression through sequential effects on the transcriptional activity of E2F-responsive promoters during G(1) and S phases.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SERTAD1 antibody 10167-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
