Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICC

WB analysis using 13801-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13801-1-AP (Serpin E1/PAI-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 13801-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 13801-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 13801-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13801-1-AP (Serpin E1/PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 13801-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13801-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HUVEC using 13801-1-AP
IP result of anti-Serpin E1/PAI-1 (IP:13801-1-AP, 4ug; Detection:13801-1-AP 1:4000) with HUVEC cells lysate 840 ug.
IHC staining of human placenta using 13801-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13801-1-AP (Serpin E1/PAI-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 13801-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13801-1-AP (Serpin E1/PAI-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human malignant melanoma using 13801-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 13801-1-AP (Serpin E1/PAI-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human malignant melanoma using 13801-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 13801-1-AP (Serpin E1/PAI-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 13801-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13801-1-AP (Serpin E1/PAI-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 13801-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13801-1-AP (Serpin E1/PAI-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13801-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13801-1-AP (Serpin E1/PAI-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13801-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13801-1-AP (Serpin E1/PAI-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13801-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13801-1-AP (Serpin E1/PAI-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13801-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13801-1-AP (Serpin E1/PAI-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 13801-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Serpin E1/PAI-1 antibody (13801-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 13801-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PAI-1 antibody (13801-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HepG2 using 13801-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Serpin E1/PAI-1 antibody (13801-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

Serpin E1/PAI-1 Polyclonal antibody

Serpin E1/PAI-1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 13801-1-AP

Publications(103)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Blocking assay

Synonyms

PAI-1, SERPINE1, SERPINE1, PAI-1, PAI, PAI 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (103)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HuH-7 cells, L02 cells, MG-63 cells, U2OS cells, human placenta tissue
Positive IP detected in	HUVEC cells
Positive IHC detected in	human placenta tissue, human lung cancer tissue, human malignant melanoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 87 publications below
IHC	See 20 publications below
IF	See 5 publications below
CoIP	See 1 publications below

Product Information

13801-1-AP targets Serpin E1/PAI-1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rabbit, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4777 Product name: Recombinant human SERPINE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-402 aa of BC010860 Sequence: MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP 相同性解析による交差性が予測される生物種
Full Name	serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Calculated molecular weight	402 aa, 45 kDa
Observed molecular weight	45 kDa
GenBank accession number	BC010860
Gene Symbol	PAI-1
Gene ID (NCBI)	5054
RRID	AB_2186881
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05121
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. In western blotting, we got two bands 50 kDa and 90-110 kDa, and 90-110 kDa consistent with PAI-1 in complex with its target protease, t-PA (PMID: 21596853).

Protocols

Product Specific Protocols
IF protocol for Serpin E1/PAI-1 antibody 13801-1-AP	Download protocol
IHC protocol for Serpin E1/PAI-1 antibody 13801-1-AP	Download protocol
IP protocol for Serpin E1/PAI-1 antibody 13801-1-AP	Download protocol
WB protocol for Serpin E1/PAI-1 antibody 13801-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Discov Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer Authors - Nurmaa K Dashzeveg View Article
human		Cell Stem Cell Vitamin C conveys geroprotection on primate ovaries Authors - Ying Jing View Article
human	WB	Am J Respir Crit Care Med CD38 Mediates Lung Fibrosis by Promoting Alveolar Epithelial Cell Aging Authors - Huachun Cui View Article
human	WB	Nat Commun ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer. Authors - Rokana Taftaf View Article
human	WB,IHC,IF	Nat Commun A translational program that suppresses metabolism to shield the genome. Authors - Nathan C Balukoff View Article
human	WB	Nat Commun A circular RNA activated by TGFβ promotes tumor metastasis through enhancing IGF2BP3-mediated PDPN mRNA stability Authors - Ke Li View Article

Validation Data Gallery

WB analysis using 13801-1-AP

WB analysis using 13801-1-AP

WB analysis using 13801-1-AP

WB analysis using 13801-1-AP

WB analysis using 13801-1-AP

WB analysis of HepG2 using 13801-1-AP

IP experiment of HUVEC using 13801-1-AP

IHC staining of human placenta using 13801-1-AP

IHC staining of human placenta using 13801-1-AP

IHC staining of human malignant melanoma using 13801-1-AP

IHC staining of human malignant melanoma using 13801-1-AP

IHC staining of human placenta using 13801-1-AP

IHC staining of human placenta using 13801-1-AP

IHC staining of human lung cancer using 13801-1-AP

IHC staining of human lung cancer using 13801-1-AP

IHC staining of human lung cancer using 13801-1-AP

IHC staining of human lung cancer using 13801-1-AP

IF Staining of mouse kidney using 13801-1-AP

IF Staining of HepG2 using 13801-1-AP

IF Staining of HepG2 using 13801-1-AP

Serpin E1/PAI-1 Polyclonal antibody

Serpin E1/PAI-1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer

Vitamin C conveys geroprotection on primate ovaries

CD38 Mediates Lung Fibrosis by Promoting Alveolar Epithelial Cell Aging

ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.

A translational program that suppresses metabolism to shield the genome.

A circular RNA activated by TGFβ promotes tumor metastasis through enhancing IGF2BP3-mediated PDPN mRNA stability

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