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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using SERPINB9 Monoclonal antibody (67950-1-Ig)

    WB analysis of K-562 using 67950-1-Ig (same clone as 67950-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using SERPINB9 Monoclonal antibody (67950-1-Ig)

    WB analysis of Daudi using 67950-1-Ig (same clone as 67950-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using SERPINB9 Monoclonal antibody (67950-1-Ig)

    WB analysis of Raji using 67950-1-Ig (same clone as 67950-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using SERPINB9 Monoclonal antibody (67950-1-Ig)

    WB analysis of Ramos using 67950-1-Ig (same clone as 67950-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using SERPINB9 Monoclonal antibody (67950-1-Ig)

    WB analysis of human placenta using 67950-1-Ig (same clone as 67950-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SERPINB9 Monoclonal antibody (67950-1-Ig)

    IHC staining of mouse lung using 67950-1-Ig (same clone as 67950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67950-1-Ig (SERPINB9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SERPINB9 Monoclonal antibody (67950-1-Ig)

    IHC staining of mouse lung using 67950-1-Ig (same clone as 67950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67950-1-Ig (SERPINB9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using SERPINB9 Monoclonal antibody (67950-1-Ig)

    IF Staining of C6 using 67950-1-Ig (same clone as 67950-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using SERPINB9 antibody (67950-1-Ig, Clone: 2H9D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

SERPINB9 Monoclonal antibody, PBS Only

SERPINB9 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67950-1-PBS
Clone No.2H9D2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CAP 3, CAP3, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI 9, PI9, Serpin B9, SERPINB9

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67950-1-PBS targets SERPINB9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6719

Product name: Recombinant human SERPINB9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-376 aa of BC002538

Sequence: METLSNASGTFAIRLLKILCQDNPSHNVFCSPVSISSALAMVLLGAKGNTATQMAQALSLNTEEDIHRAFQSLLTEVNKAGTQYLLRTANRLFGEKTCQFLSTFKESCLQFYHAELKELSFIRAAEESRKHINTWVSKKTEGKIEELLPGSSIDAETRLVLVNAIYFKGKWNEPFDETYTREMPFKINQEEQRPVQMMYQEATFKLAHVGEVRAQLLELPYARKELSLLVLLPDDGVELSTVEKSLTFEKLTAWTKPDCMKSTEVEVLLPKFKLQEDYDMESVLRHLGIVDAFQQGKADLSAMSAERDLCLSKFVHKSFVEVNEEGTEAAAASSCFVVAECCMESGPRFCADHPFLFFIRHNRANSILFCGRFSSP

相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 9
Calculated molecular weight 42 kDa
Observed molecular weight38 kDa
GenBank accession numberBC002538
Gene Symbol SERPINB9
Gene ID (NCBI) 5272
RRIDAB_2918702
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP50453
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SERPINB9, also named as PI-9, belongs to the serpin family. SERPINB9 is an intracellular inhibitor of granzyme B (grB) that protects cytotoxic lymphocytes from grB-mediated death. It has a nucleo-cytoplasmic distribution and is produced in CD8+ T cells and natural killer cells (PMID: 28024184). Also, SERPINB9 is expressed by vascular SMCs and endothelial cells to avoid GZB-induced apoptosis (PMID: 28743062). The molecular weight of SERPINB9 is 42 kDa.

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