Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse liver tissue, rat liver tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21170-1-AP targets SERPINB7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15394 Product name: Recombinant human SERPINB7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 32-305 aa of BC069417 Sequence: SLSLFAALALVRLGAQDDSLSQIDKLLHVNTASGYGNSSNSQSGLQSQLKRVFSDINASHKDYDLSIVNGLFAEKVYGFHKDYIECAEKLYDAKVERVDFTNHLEDTRRNINKWVENETHGKIKNVIGEGGISSSAVMVLVNAVYFKGKWQSAFTKSETINCHFKSPKCSGKAVAMMHQERKFNLSVIEDPSMKILELRYNGGINMYVLLPENDLSEIENKLTFQNLMEWTNPRRMTSKYVEVFFPQFKIEKNYEMKQYLRALGLKDIFDESKA 相同性解析による交差性が予測される生物種 |
| Full Name | serpin peptidase inhibitor, clade B (ovalbumin), member 7 |
| Calculated molecular weight | 380 aa, 43 kDa |
| Observed molecular weight | 43-45 kDa |
| GenBank accession number | BC069417 |
| Gene Symbol | SERPINB7 |
| Gene ID (NCBI) | 8710 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75635 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Serine protease inhibitor B7 (SERPINB7) is also named Serpin B7, Megsin and TP55. SerpinB7 is identified as a skin-specific endogenous protease inhibitor. SerpinB7 is upregulated in diabetic nephropathy and induces progressive mesangial expansion (PMID: 11877466). SERPINB7 is abundantly expressed in keratinocytes, with particularly high levels in the stratum corneum and stratum granulosum of the epidermis (PMID: 41469525). SERPINB7 promotes keratinocyte differentiation and dampens inflammation in psoriasis in a calcium-dependent manner (PMID: 35864103). SERPINB7 is expressed mostly in renal mesangial cells, and its expression is significantly increased in renal tissues of IgA and diabetic nephropathies. SERPINB7 regulates cell proliferation, signal transduction, and extracellular matrix metabolism (PMID: 37809412).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SERPINB7 antibody 21170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |