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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using SERPINA7/ TBG Monoclonal antibody (66454-1-Ig)

    WB analysis of human plasma using 66454-1-Ig (same clone as 66454-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66454-1-Ig (SERPINA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66454-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using SERPINA7/ TBG Monoclonal antibody (66454-1-Ig)

    WB analysis of human testis using 66454-1-Ig (same clone as 66454-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66454-1-Ig (SERPINA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SERPINA7/ TBG Monoclonal antibody (66454-1-Ig)

    IHC staining of human liver cancer using 66454-1-Ig (same clone as 66454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SERPINA7/ TBG Monoclonal antibody (66454-1-Ig)

    IHC staining of human liver cancer using 66454-1-Ig (same clone as 66454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SERPINA7/ TBG Monoclonal antibody (66454-1-Ig)

    IHC staining of mouse liver using 66454-1-Ig (same clone as 66454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SERPINA7/ TBG Monoclonal antibody (66454-1-Ig)

    IHC staining of mouse liver using 66454-1-Ig (same clone as 66454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66454-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using SERPINA7/ TBG Monoclonal antibody (66454-1-Ig)

    IF Staining of human liver cancer using 66454-1-Ig (same clone as 66454-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERPINA7 antibody (66454-1-Ig, Clone: 1B11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66454-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using SERPINA7/ TBG Monoclonal antibody (66454-1-Ig)

    IF Staining of human liver cancer using 66454-1-Ig (same clone as 66454-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERPINA7 antibody (66454-1-Ig, Clone: 1B11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66454-1-PBS in a different storage buffer formulation.

SERPINA7/ TBG Monoclonal antibody, PBS Only

SERPINA7/ TBG Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66454-1-PBS
Clone No.1B11B10

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

SERPINA7, 1B11B10, Serpin A7, SERPINA 7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66454-1-PBS targets SERPINA7/ TBG in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9823

Product name: Recombinant human SERPINA7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 38-415 aa of BC020747

Sequence: TLYKMSSINADFAFNLYRRFTVETPDKNIFFSPVSISAALVMLSFGACCSTQTEIVETLGFNLTDTPMVEIQHGFQHLICSLNFPKKELELQIGNALFIGKHLKPLAKFLNDVKTLYETEVFSTDFSNISAAKQEINSHVEMQTKGKVVGLIQDLKPNTIMVLVNYIHFKAQWANPFDPSKTEDSSSFLIDKTTTVQVPMMHQMEQYYHLVDMELNCTVLQMDYSKNALALFVLPKEGQMESVEAAMSSKTLKKWNRLLQKGWVDLFVPKFSISATYDLGATLLKMGIQHAYSENADFSGLTEDNGLKLSNAAHKAVLHIGEKGTEAAAVPEVELSDQPENTFLHPIIQIDRSFMLLILERSTRSILFLGKVVNPTEA

相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
Calculated molecular weight 415 aa, 46 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC020747
Gene Symbol SERPINA7
Gene ID (NCBI) 6906
RRIDAB_2881823
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP05543
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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