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Validation Data Gallery

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  • Western Blot (WB) analysis of human adipose tissue using Vaspin Polyclonal antibody (17451-1-AP)

    WB analysis of human adipose using 17451-1-AP

    human adipose tissue were subjected to SDS PAGE followed by western blot with 17451-1-AP (SERPINA12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Serpin A12/Vaspin Polyclonal antibody

Serpin A12/Vaspin Polyclonal Antibody for WB, ELISA
Cat No. 17451-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, ELISA

Synonyms

SERPINA12, SERPINA12/Vaspin, Vaspin, OL 64, OL-64

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman adipose tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17451-1-AP targets Serpin A12/Vaspin in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Serpin A12/Vaspin fusion protein Ag11574 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
Calculated molecular weight 414 aa, 47 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC040857
Gene Symbol Vaspin
Gene ID (NCBI) 145264
RRIDAB_2878408
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IW75
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINA12 is also named as Serpin A12 and Visceral adipose tissue-derived serine protease inhibitor (Vaspin). Vaspin is an adipokine belonging to the serpin family, thus also named SERPINA12, with targets in kallikrein 7 and 14 that participates in skin desquamation (PMID: 34359881). Vaspin is a compensatory molecule in obesity and insulin resistance (PMID: 18321232). Vaspin is a newly described adipokine with an insulin sensitizing effect and inhibitory impact on food intake (PMID: 16030142, PMID: 21465327).

Protocols

Product Specific Protocols
WB protocol for Serpin A12/Vaspin antibody 17451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat,mouseWB

Biochem Biophys Res Commun

Vaspin alleviates myocardial ischaemia/reperfusion injury via activating autophagic flux and restoring lysosomal function.

Authors - Feihong Yang
View Article