Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12725-1-AP targets SERF1A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3422 Product name: Recombinant human SERF1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-62 aa of BC021174 Sequence: MARGNQRELARQKNMKKTQEISKGKRKEDSLTASQRKQRDSEIMQEKQKAANEKKSMQTREK 相同性解析による交差性が予測される生物種 |
| Full Name | small EDRK-rich factor 1A (telomeric) |
| Calculated molecular weight | 62 aa, 7 kDa |
| Observed molecular weight | 12-15 kDa |
| GenBank accession number | BC021174 |
| Gene Symbol | SERF1A |
| Gene ID (NCBI) | 8293 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75920 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Small EDRK-rich factor 1 (SERF1A) is also named SMA modifier 1 (SMAM1). SERF1a is part of a 500 kb inverted duplication on chromosome 5q13 and highly related to spinal muscular atrophy. Human SERF1a protein has two isoforms. The short form contains 62 residues, and the long form has 110 amino acids. They share a highly conserved N-terminal region, and they are enriched in lysine and arginine (PMID: 37479809). SERF1a was found to promote amyloid formation in vitro on several amyloid proteins, including α-synuclein, huntingtin, Aβ, and prion proteins (PMID: 22854022). SERF1a has been reported as an RNA-binding protein capable of mediating the functional integration of RNA in the nucleolus (PMID: 31794729). This target may exhibit cross-reaction with SERF2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SERF1A antibody 12725-1-AP | Download protocol |
| WB protocol for SERF1A antibody 12725-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |