Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, MDCK cells, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67792-1-Ig targets SERBP1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, pig, Canine samples.
| Tested Reactivity | Human, Mouse, Rat, pig, Canine |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30202 Product name: Recombinant human SERBP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 108-408 aa of BC008045 Sequence: RVGRRPDQQLQGEGKIIDRRPERRPPRERRFEKPLEEKGEGGEFSVDRPIIDRPIRGRGGLGRGRGGRGRGMGRGDGFDSRGKREFDRHSGSDRSSFSHYSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNYSDLDQSNVTEETPEGEEHHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEFNIRKPNEGADGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINFGDLGRPGRGGRGGRGGRGRGGRPNRGSRTDKSSASAPDVDDPEAFPALA 相同性解析による交差性が予測される生物種 |
| Full Name | SERPINE1 mRNA binding protein 1 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC008045 |
| Gene Symbol | SERBP1 |
| Gene ID (NCBI) | 26135 |
| RRID | AB_2918556 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8NC51 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SERBP1 antibody 67792-1-Ig | Download protocol |
| IHC protocol for SERBP1 antibody 67792-1-Ig | Download protocol |
| IP protocol for SERBP1 antibody 67792-1-Ig | Download protocol |
| WB protocol for SERBP1 antibody 67792-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
