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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERAC1 Polyclonal antibody (25729-1-AP)

    WB analysis using 25729-1-AP (same clone as 25729-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25729-1-AP (SERAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25729-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SERAC1 Polyclonal antibody (25729-1-AP)

    WB analysis of mouse brain using 25729-1-AP (same clone as 25729-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25729-1-AP (SERAC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25729-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using SERAC1 Polyclonal antibody (25729-1-AP)

    IHC staining of human colon using 25729-1-AP (same clone as 25729-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25729-1-AP (SERAC1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25729-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using SERAC1 Polyclonal antibody (25729-1-AP)

    IHC staining of human colon using 25729-1-AP (same clone as 25729-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25729-1-AP (SERAC1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25729-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SERAC1 Polyclonal antibody (25729-1-AP)

    IF Staining of HepG2 using 25729-1-AP (same clone as 25729-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 25729-1-AP (SERAC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25729-1-PBS in a different storage buffer formulation.

SERAC1 Polyclonal antibody, PBS Only

SERAC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25729-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Serine active site-containing protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25729-1-PBS targets SERAC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22790

Product name: Recombinant human SERAC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-162 aa of BC001705

Sequence: MSLAAYCVICCRRIGTSTSPPKSGTHWRDIRNIIKFTGSLILGGSLFLTYEVLALKKAVTLDTQVVEREKMKSYIYVHTVSLDKGENHGIAWQARKELHKAVRKVLATSAKILRNPFADPFSTVDIEDHECAVWLLLRKSKSDDKTTRLEAVREMSETHHWH

相同性解析による交差性が予測される生物種
Full Name serine active site containing 1
Calculated molecular weight 654 aa, 74 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC001705
Gene Symbol SERAC1
Gene ID (NCBI) 84947
RRIDAB_2880213
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JX3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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