Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 11769-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 11769-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11769-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 11769-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11769-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11769-1-AP
IP result of anti-Septin 8 (IP:11769-1-AP, 3ug; Detection:11769-1-AP 1:1000) with HeLa cells lysate 2000ug.
IHC staining of human gliomas using 11769-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (Septin 8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11769-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (SEPT8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 11769-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11769-1-AP (Septin 8 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 11769-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11769-1-AP (SEPT8 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human gliomas using 11769-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (Septin 8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11769-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (SEPT8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of hTERT-RPE1 using 11769-1-AP
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Septin 8 antibody (11769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

Septin 8 Polyclonal antibody

Septin 8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11769-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

SEPT8, Septin-8, SEPTIN8, SEP2, KIAA0202

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse testis tissue, K-562 cells, mouse brain tissue, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human gliomas tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	hTERT-RPE1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 10 publications below
IF	See 5 publications below

Product Information

11769-1-AP targets Septin 8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2357 Product name: Recombinant human SEPT8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-256 aa of BC001329 Sequence: MKKLDSKVNIIPIIAKADTISKSELHKFKIKIMGELVSNGVQIYQFPTDDEAVAEINAVMNAHLPFAVVGSTEEVKVGNKLVRARQYPWGVVQVENENHCDFVKLREMLIRVNMEDLREQTHSRHYELYRRCKLEEMGFQDSDGDSQPFSLQETYEAKRKEFLSELQRKEEEMRQMFVNKVKETELELKEKERELHEKFEHLKRVHQEEKRKVEEKRRELEEETNAFNRRKAAVEALQSQALHATSQQPLRKDKDK 相同性解析による交差性が予測される生物種
Full Name	septin 8
Calculated molecular weight	55.6 kDa
Observed molecular weight	50-56 kDa
GenBank accession number	BC001329
Gene Symbol	Septin 8
Gene ID (NCBI)	23176
RRID	AB_2185006
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92599
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are 40-60kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking. It may play a role in platelet secretion. Septin 8 is about 50-56 kDa in WB detection.

Protocols

Product Specific Protocols
IF protocol for Septin 8 antibody 11769-1-AP	Download protocol
IHC protocol for Septin 8 antibody 11769-1-AP	Download protocol
IP protocol for Septin 8 antibody 11769-1-AP	Download protocol
WB protocol for Septin 8 antibody 11769-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Elife Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction. Authors - Julia Patzig View Article
mouse	WB,IF	Thromb Haemost Impaired Platelet Function in Sept8-Deficient Mice In Vitro Authors - Katharina Neubauer View Article
mouse	WB	Glia Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome Authors - Sophie B Siems View Article
		Glia The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2. Authors - Andrew A Jarjour View Article
mouse	WB	Development Pinch2 regulates myelination in the mouse central nervous system. Authors - Joana Paes de Faria View Article
mouse, human	WB	J Cell Sci SEPT8 modulates β-amyloidogenic processing of APP by affecting the sorting and accumulation of BACE1. Authors - Kaisa M A Kurkinen KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 11769-1-AP

WB analysis of mouse testis using 11769-1-AP

WB analysis of K-562 using 11769-1-AP

WB analysis of rat brain using 11769-1-AP

WB analysis of mouse brain using 11769-1-AP

IP experiment of HeLa using 11769-1-AP

IHC staining of human gliomas using 11769-1-AP

IHC staining of human gliomas using 11769-1-AP

IHC staining of human heart using 11769-1-AP

IHC staining of human heart using 11769-1-AP

IHC staining of human gliomas using 11769-1-AP

IHC staining of human gliomas using 11769-1-AP

IF Staining of hTERT-RPE1 using 11769-1-AP

Septin 8 Polyclonal antibody

Septin 8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction.

Impaired Platelet Function in Sept8-Deficient Mice In Vitro

Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome

The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2.

Pinch2 regulates myelination in the mouse central nervous system.

SEPT8 modulates β-amyloidogenic processing of APP by affecting the sorting and accumulation of BACE1.