Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of Jurkat using 11631-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 11631-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of U2OS using 11631-1-AP
WB result of Septin 5 antibody (11631-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 5 transfected U2OS cells.
WB analysis of human brain using 11631-1-AP
Human brain tissue were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 11631-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11631-1-AP
IP result of anti-Septin 5 (IP:11631-1-AP, 3ug; Detection:11631-1-AP 1:300) with mouse brain tissue lysate 1000ug.
IHC staining of human gliomas using 11631-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11631-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 11631-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 11631-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 11631-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

Septin 5 Polyclonal antibody

Septin 5 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11631-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

SEPT5, CDCREL, CDCREL 1, CDCREL1, CDCrel-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, human brain tissue, NIH/3T3 cells, mouse brain tissue, U2OS cells, rat brain tissue, K-562 cells, LNCaP cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human gliomas tissue, mouse cerebellum tissue, human placenta tissue, rat cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 9 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

11631-1-AP targets Septin 5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2205 Product name: Recombinant human SEPT5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-369 aa of BC025261 Sequence: MSTGLRYKSKLATPEDKQDIDKQYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVHSLFLTDLYKDRKLLSAEERISQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKPITDYVDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVGFMKALHEKVNIVPLIAKADCLVPSEIRKLKERIREEIDKFGIHVYQFPECDSDEDEDFKQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVEVENQAHCDFVKLRNMLIRTHMHDLKDVTCDVHYENYRAHCIQQMTSKLTQDSRMESPIPILPLPTPDAETEKLIRMKDEELRRMQEMLQRMKQQMQDQ 相同性解析による交差性が予測される生物種
Full Name	septin 5
Calculated molecular weight	369 aa, 43 kDa
Observed molecular weight	40-43 kDa
GenBank accession number	BC025261
Gene Symbol	Septin 5
Gene ID (NCBI)	5413
RRID	AB_2301692
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99719
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Septin 5, a septin family member formerly called cell division control related protein 1 (CDCrel), is encoded in the 200 kb region and is abundantly expressed in the developing and adult brains of mice and humans. Although its constitutive deletion does not cause detectible structural or developmental abnormalities in the mouse brain, Septin 5 has been implicated in subtle neuronal and behavioral functions. Septin 5 is expressed in presynaptic terminals and exerts negative regulatory control over neurotransmitter release by binding to SNARE-protein, which has been implicated in neuropsychiatric disorders (PMID: 19240081). Septin 5 has 2 isoforms with the molecular mass of 39 and 43 kDa.

Protocols

Product Specific Protocols
IHC protocol for Septin 5 antibody 11631-1-AP	Download protocol
IP protocol for Septin 5 antibody 11631-1-AP	Download protocol
WB protocol for Septin 5 antibody 11631-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat,mouse,human	WB,IF	Nat Commun Septin5 deletion enhances β-cell exocytosis by releasing microtubule-tethered insulin granules onto plasma membrane Authors - Li Xie View Article
rat,mouse,human	WB	J Adv Res Pharmacological inhibition of Septins with Forchlorfenuron attenuates thrombus formation in experimental thrombotic mice models with modulating multiple signaling pathways in platelets Authors - Zhen Hao View Article
human	WB,IF	Cells Presynaptic Vesicle Protein SEPTIN5 Regulates the Degradation of APP C-Terminal Fragments and the Levels of Aβ. Authors - Mikael Marttinen KO Validated View Article
mouse	WB	Neurobiol Dis S327 phosphorylation of the presynaptic protein SEPTIN5 increases in the early stages of neurofibrillary pathology and alters the functionality of SEPTIN5. Authors - Catarina B Ferreira View Article
rat	WB	Molecules Synergistic Neuroprotective Effect of Endogenously-Produced Hydroxytyrosol and Synaptic Vesicle Proteins on Pheochromocytoma Cell Line Against Salsolinol. Authors - Robina Manzoor View Article
human	WB	Cancers (Basel) Forchlorfenuron-Induced Mitochondrial Respiration Inhibition and Metabolic Shifts in Endometrial Cancer Authors - Kyukwang Kim View Article

Validation Data Gallery

WB analysis of Jurkat using 11631-1-AP

WB analysis using 11631-1-AP

WB analysis of U2OS using 11631-1-AP

WB analysis of human brain using 11631-1-AP

WB analysis of NIH/3T3 using 11631-1-AP

IP experiment of mouse brain using 11631-1-AP

IHC staining of human gliomas using 11631-1-AP

IHC staining of human gliomas using 11631-1-AP

IHC staining of mouse cerebellum using 11631-1-AP

IHC staining of human placenta using 11631-1-AP

IHC staining of rat cerebellum using 11631-1-AP

Septin 5 Polyclonal antibody

Septin 5 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Septin5 deletion enhances β-cell exocytosis by releasing microtubule-tethered insulin granules onto plasma membrane

Pharmacological inhibition of Septins with Forchlorfenuron attenuates thrombus formation in experimental thrombotic mice models with modulating multiple signaling pathways in platelets

Presynaptic Vesicle Protein SEPTIN5 Regulates the Degradation of APP C-Terminal Fragments and the Levels of Aβ.

S327 phosphorylation of the presynaptic protein SEPTIN5 increases in the early stages of neurofibrillary pathology and alters the functionality of SEPTIN5.

Synergistic Neuroprotective Effect of Endogenously-Produced Hydroxytyrosol and Synaptic Vesicle Proteins on Pheochromocytoma Cell Line Against Salsolinol.

Forchlorfenuron-Induced Mitochondrial Respiration Inhibition and Metabolic Shifts in Endometrial Cancer

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