Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse testis tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13707-1-AP targets SEPT12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SEPT12 fusion protein Ag4644 相同性解析による交差性が予測される生物種 |
| Full Name | septin 12 |
| Calculated molecular weight | 358 aa, 41 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC035619 |
| Gene Symbol | SEPT12 |
| Gene ID (NCBI) | 124404 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IYM1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SEPT12, also known as Septin 12, SPGF10, is a member of the Septin GTPase family. The Septin family is a group of evolutionarily conserved GTP-binding proteins involved in various cellular processes. SEPT12 is a cytoskeletal GTPase that is specifically enriched in the testis. It plays a critical role in spermatogenesis, particularly in the formation and maintenance of sperm tail structures. Mutations or downregulation of SEPT12 have been linked to male infertility due to defects in sperm morphology and function(PMID: 30513371).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SEPT12 antibody 13707-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |