Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human ovary tissue, human lung tissue, rat spleen tissue, mouse liver tissue, mouse stomach tissue, human spleen tissue, human kidney tissue, rat liver tissue, human stomach cancer tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
14672-1-AP targets Septin 11 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6342 Product name: Recombinant human SEPT11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC063615 Sequence: MAVAVGRPSNEELRNLSLSGHVGFDSLPDQLVNKSTSQGFCFNILCVGETGIGKSTLMDTLFNTKFESDPATHNEPGVRLKARSYELQESNVRLKLTIVDTVGFGDQINKDDSYKPIVEYIDAQFEAYLQEELKIKRSLFNYHDTRIHAC 相同性解析による交差性が予測される生物種 |
| Full Name | septin 11 |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC063615 |
| Gene Symbol | Septin 11 |
| Gene ID (NCBI) | 55752 |
| RRID | AB_2301682 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NVA2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| IF protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| IHC protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| IP protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| WB protocol for Septin 11 antibody 14672-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942. | ||
Nat Commun Quantitative determination of the spatial distribution of components in single cells with CellDetail | ||
Brain Septin multimer autoantibodies in severe motor neuropathy mimicking lower motor neuron disease. | ||
Cell Death Dis Septin11 promotes hepatocellular carcinoma cell motility by activating RhoA to regulate cytoskeleton and cell adhesion | ||
Cell Rep An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis | ||
Cancers (Basel) Forchlorfenuron-Induced Mitochondrial Respiration Inhibition and Metabolic Shifts in Endometrial Cancer |